Efficient RNA-Based Reprogramming of Disease-Associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells.
Efficient RNA-Based Reprogramming of Disease-Associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells.
复制标题
将与疾病相关的原代人成纤维细胞进行基于 RNA 的有效重编程为诱导多能干细胞。
DOI：
10.1007/978-1-0716-0301-7_17
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Bilousova,Ganna
中科院分区：
文献类型：
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作者：
McGrath,PatrickS;McGarvey,ShenneaS;Kogut,Igor;Bilousova,Ganna
Reprogramming a patient’s somatic cells into induced pluripotent stem cells (iPSCs) holds great promise for disease modeling and the development of autologous cellular therapeutics. However, it remains challenging to consistently reprogram primary human cells, as they are frequently aged, diseased, or in low abundance. Here we present a modified highly efficient and clinically relevant RNA-based method for reprogramming disease-associated and other difficult-to-reprogram human primary fibroblast lines into iPSCs. We also describe optimizations that can be employed for consistent reprogramming of these difficult-to-reprogram cells. With the provided protocol, integration-free iPSC lines can be successfully generated from a small number of primary human fibroblasts in approximately 5–7 weeks.
