Quantitative measurements of the optokinetic response in adult fish
Quantitative measurements of the optokinetic response in adult fish
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DOI：
10.1016/j.jneumeth.2009.10.020
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发表时间：
2010-01-30
影响因子：
3
通讯作者：
Neuhauss, Stephan C. F.
中科院分区：
文献类型：
--
作者：
Mueller, Kaspar P.;Neuhauss, Stephan C. F.
小型硬骨鱼越来越多地用于研究视觉的遗传基础。特别是斑马鱼（Danio rerio）和青鳉（Oryzias latipes）是发育研究中常用的脊椎动物模式生物，包括视觉功能发育的研究。许多基于行为的视觉测试已成功地用于识别和表征这些物种的遗传操作菌株中的视觉缺陷的幼虫。测试成鱼的视觉系统已被证明是更困难的原因有很多，包括在约束鱼的并发症，或浅滩和优势行为干扰种群筛选试验中的视觉行为。本文提出了一种简单有效的方法来定量测量个体成年斑马鱼和青鳉的视动反应（OKR），它可以用来表征成鱼的视觉能力。这种方法适用于任何大小相似的鱼类。(C)2009 Elsevier B.V.保留所有权利。
Small teleost fish are increasingly used for studying the genetic basis of vision. In particular, zebrafish (Danio rerio) and medaka (Oryzias latipes) are commonly used vertebrate model organisms in developmental research, including research on the development of visual function. A multitude of behavior-based visual tests are established for larvae that have been successfully used to identify and characterize visual defects in genetically manipulated strains of these species. Testing the visual system of adult fish has proven to be more difficult for a number of reasons, including complications in restraining fish, or shoaling and dominance behavior interfering with visual behavior in population screening assays. In this paper, we present a simple and cost-effective method to quantitatively measure the optokinetic response (OKR) of individual adult zebrafish and medaka, which can be used to characterize visual capabilities of adult fish. This method can be applied to any fish species of similar size. (C) 2009 Elsevier B.V. All rights reserved.