Chromogenic in situ hybridization for the detection of HER-2/neu gene amplification in breast cancer with an emphasis on tumors with borderline and low-level amplification -: Does it measure up to fluorescence in situ hybridization?
Chromogenic in situ hybridization for the detection of HER-2/neu gene amplification in breast cancer with an emphasis on tumors with borderline and low-level amplification -: Does it measure up to fluorescence in situ hybridization?
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DOI：
10.1309/c4pebgb9ln830tvl
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发表时间：
2005-02-01
影响因子：
3.5
通讯作者：
Chen, BY
中科院分区：
文献类型：
--
作者：
Bhargava, R;Lal, P;Chen, BY
我们用福尔马林固定、石蜡包埋的组织芯片制作的组织芯片(TMA)对113例浸润性乳腺癌的HER-2/neu基因扩增进行了显色原位杂交和荧光原位杂交的比较。在两种检测方法中，均使用0.6 mm的组织核心和每个肿瘤4个采样核心来创建TMA，HER-2/17号染色体信号比率等于或大于2.0被认为是基因扩增的阳性。用同一肿瘤的平均核心率来确定该特定肿瘤的基因扩增状态113例，两种方法都成功地检测了102例。结果符合率为100.0%(扩增63例，未扩增39例)。22例临界性(比值2.0~2.5)或低水平(比值2.6~3.9)FISH扩增均为HER-2基因扩增。在检测临界性和低水平HER-2扩增方面，CISH与FISH一样敏感。通过光学显微镜可靠地识别浸润性癌区域和保存测试玻片是CISH的另一个优点。在乳腺癌HER-2基因扩增的分析中，CISH的表现与FISH一样好，在某些情况下可能具有优势。
We compared chromogenic in situ hybridization (CISH) with fluorescence in situ, hybridization (FISH) for assessing HER-2/neu gene amplification using tissue microarrays (TMAs) made from formalin-fixed, paraffin-embedded tissue blocks,from 113 cases of invasive breast carcinoma. TMAs were created using 0.6-mm tissue cores with 4 sampled cores per tumor For both assays, a HER-2/chromosome 17 signal ratio of 2.0 or more was considered positive for gene amplification. The average ratio of cores from the same tumor was used for determination of gene amplification status of that particular tumor Of 113 cases, 102 were tested successfully by both assays. The results were concordant in 100.0% of cases (63 amplified; 39 nonamplified). All 22 cases of borderline (ratio, 2.0-2.5) or low-level (ratio, 2.6-3.9) amplification by FISH also showed HER-2 gene amplification by CISH. CISH is as sensitive as FISH in detecting borderline and low-level HER-2 amplification. Reliable recognition of the invasive carcinoma area by light microscopy and preservation of the test slides are added advantages of CISH. CISH performs as well as FISH in the analysis of HER-2 gene amplification in breast cancer and might have advantages in certain situations.