Regulation of myelination: axons not required for the biosynthesis of basal levels of the major myelin glycoprotein by Schwann cells in denervated distal segments of the adult cat sciatic nerve.
Regulation of myelination: axons not required for the biosynthesis of basal levels of the major myelin glycoprotein by Schwann cells in denervated distal segments of the adult cat sciatic nerve.
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髓鞘形成的调节：成年猫坐骨神经去神经末节中的雪旺细胞生物合成基础水平的主要髓磷脂糖蛋白不需要轴突。
DOI：
10.1002/jnr.490140203
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发表时间：
1985
影响因子：
4.2
通讯作者：
Spencer,PS
中科院分区：
文献类型：
--
作者：
Poduslo,JF;Berg,CT;Ross,SM;Spencer,PS
成年猫坐骨神经永久性神经切断后，在远端段，没有轴突再生或髓鞘组装的主要髓鞘糖蛋白（P0）的雪旺细胞生物合成进行了检查。将离断后10周脱鞘猫坐骨神经远端段和正常成人脱鞘臂神经的神经内膜切片（束内组织）与放射性甘露糖一起孵育;十二烷基硫酸钠-孔梯度电泳（SDS-PGE）后，通过荧光照相术观察[3 H]甘露糖掺入P0。用兔抗鸡P0 γ球蛋白孵育一等份神经内膜组分后，通过SDS-PGE分析免疫沉淀物，证实[3 H]甘露糖标记的糖蛋白为P0。在成年猫的正常臂神经中，[3 H]甘露糖掺入P0和其他神经内膜糖蛋白的水平与横断神经相比大幅降低。只有在长时间暴露于X射线胶片（15天）后，才能通过荧光照相术检测到这种掺入。因此，在正常成年猫的P0的生物合成的水平大大降低，这表明在成年猫神经的活跃髓鞘形成的程度是在一个低水平。此外，雪旺氏细胞能够继续合成P0在成人，永久横断的神经轴突的影响的情况下，这表明轴突协会是不是一个绝对的要求，指定髓鞘蛋白质的合成。
The adult cat sciatic nerve was examined for Schwann cell biosynthesis of the major myelin glycoprotein (P0) in the distal segments after permanent nerve transection, where there is no axonal regeneration or myelin assembly. Endoneurial slices (intrafascicular tissue) from the distal segment of the desheathed cat sciatic nerves at 10 wk after transection and from normal adult desheathed brachial nerves were incubated with radioactive mannose; [3H]mannose incorporation into P0was observed by fluorography after sodium dodecyl sulphate‐pore gradient electrophoresis (SDS‐PGE). Analysis of immune precipitates by SDS‐PGE after incubation of an aliquot of an endoneurial fraction with rabbit antichick P0gamma globulin verified that the [3H]mannose‐labeled glycoprotein was P0. The level of incorporation of [3H]mannose into P0and into other endoneurial glycoproteins in the normal brachial nerve from the adult cat was at substantially reduced levels compared with the transected nerve. Such incorporation was detectable by fluorography only after prolonged exposure to X‐ray film (15 days). As a result, the level of biosynthesis of P0in the normal adult cat is substantially reduced, suggesting that the extent of active myelination in the adult cat nerve is at a low level. Furthermore, Schwann cells are capable of continued synthesis of P0in the adult, permanently transected nerve in the absence of axonal influence, suggesting that axonal association is not an absolute requirement for specifying myelin protein synthesis.