UV-C radiation induces apoptotic-like changes in Arabidopsis thaliana
UV-C radiation induces apoptotic-like changes in Arabidopsis thaliana
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DOI：
10.1016/s0014-5793(98)01208-3
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发表时间：
1998-10-16
期刊：
影响因子：
3.5
通讯作者：
Gallois, P
中科院分区：
文献类型：
--
作者：
Danon, A;Gallois, P
为了在分子水平上研究植物细胞的程序性死亡，我们首次报道了植物细胞核受紫外线辐射诱导的类凋亡性变化。在拟南芥幼苗中，10-50kJ/m(2)的UV-C诱导了寡核体DNA片段化，这与动物细胞中描述的DNA梯状条带相似，在UV-C处理后2小时，原生质体细胞核中也检测到这种DNA片段化。此外，UV-C还诱导了具有动物凋亡核特征的核形态。我们认为，UV-C诱导是比较植物和动物对不可逆紫外线损伤的细胞反应以及研究拟南芥中细胞程序性死亡的有力工具，(C)1998欧洲生化学会联合会，
With a view to studying programmed cell death in plants at the molecular level, we report here for the first time that apoptotic-like changes are induced by UV radiation in plant nuclei. In Arabidopsis thaliana seedlings a UV-C dose of 10-50 kJ/m(2) induces an oligonucleosomal DNA fragmentation which is reminiscent of the apoptotic DNA ladder described in animal cells, This DNA fragmentation was also detected in situ in protoplast nuclei as soon as 2 h after UV-C treatment. Moreover, UV-C induced a nuclear morphology characteristic of animal apoptotic nuclei. We propose that UV-C induction constitutes a powerful tool to compare the cellular response to irreversible UV damage in plants to that in animals and to study programmed cell death in A. thaliana, (C) 1998 Federation of European Biochemical Societies,