Low frequency of rearrangements of the ret and trk proto-oncogenes in Japanese thyroid papillary carcinomas.
Low frequency of rearrangements of the ret and trk proto-oncogenes in Japanese thyroid papillary carcinomas.
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DOI：
10.1111/j.1349-7006.1992.tb01963.x
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发表时间：
1992-07
期刊：
影响因子：
--
通讯作者：
Takahashi M
中科院分区：
文献类型：
--
作者：
Wajjwalku W;Nakamura S;Hasegawa Y;Miyazaki K;Satoh Y;Funahashi H;Matsuyama M;Takahashi M
我们研究了日本甲状腺肿瘤中ret和trk原癌基因重排的频率。用Southern印迹法分析了38例甲状腺乳头状癌和14例滤泡性腺瘤的DNA。在1例和2例乳头状癌中分别检测到ret和trk原癌基因重排，但在滤泡性腺瘤中未检测到。通过逆转录酶-聚合酶链反应方法的分析显示，ret重排阳性肿瘤含有PTC/refTPC嵌合转录物，据报道，该转录物在甲状腺肿瘤和腺瘤性甲状腺肿中特异性发现。我们还发现，在两个trk重排阳性肿瘤中的一个中，trk原癌基因的重排mRNA以高水平表达。我们的研究结果表明，日本乳头状癌中这些原癌基因重排的频率远低于意大利患者。
We investigated the frequency of rearrangements of the ret and trk proto‐oncogenes in Japanese thyroid tumors. DNAs from 38 thyroid papillary carcinomas and 14 follicular adenomas were analyzed by Southern blotting. Rearrangements of the ret and trk proto‐oncogenes were detected in one and two papillary carcinomas, respectively, but not in follicular adenomas. Analysis by a reverse transcriptase‐polymerase chain reaction method showed that the ret rearrangement‐positive tumor contained the PTC/refTPCchimeric transcript, which was reported to be found specifically in thyroid tumors and adenomatous goiter. We also found that rearranged mRNA of the trk proto‐oncogene was expressed at high levels in one of two trk rearrangement‐positive tumors. Our results indicated that the frequency of rearrangements of these proto‐oncogenes in Japanese papillary carcinomas was much lower than that in Italian patients.