Budding yeast chromosome structure and dynamics during mitosis.
Budding yeast chromosome structure and dynamics during mitosis.
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发芽的酵母染色体结构和有丝分裂过程中的动力学。
DOI：
10.1083/jcb.152.6.1255
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发表时间：
2001-03-19
影响因子：
7.8
通讯作者：
Bloom, K
中科院分区：
文献类型：
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作者：
Pearson, C G;Maddox, P S;Salmon, E D;Bloom, K
利用绿色荧光蛋白探针和快速采集的高分辨率荧光图像，发现出芽酵母的姐妹着丝粒在B后期纺锤体伸长之前分离并在纺锤极之间振荡。在这些振荡期间的运动速率与微管加端动力学相似。后期前分离的程度差异很大，在后期前观察到的着丝粒重新关联很少。着丝粒呈中期样构象，而染色体臂在后期之前既不排列也不分离。纺锤体伸长后，所有着丝粒的着丝粒向极的运动（后期A）是同步的，与纺锤体极分离同时发生或紧接着发生（后期B）。着丝粒近端染色质在后期开始前后向两极方向伸展。观察到拉伸的染色质向纺锤体极体分离的速度大于着丝粒向极体移动的速度，表明染色体臂和着丝粒之间有快速的弹性反冲。这些结果表明，DNA的弹性特性在染色体臂的极向运动中起着尚未被发现的作用。
Using green fluorescent protein probes and rapid acquisition of high-resolution fluorescence images, sister centromeres in budding yeast are found to be separated and oscillate between spindle poles before anaphase B spindle elongation. The rates of movement during these oscillations are similar to those of microtubule plus end dynamics. The degree of preanaphase separation varies widely, with infrequent centromere reassociations observed before anaphase. Centromeres are in a metaphase-like conformation, whereas chromosome arms are neither aligned nor separated before anaphase. Upon spindle elongation, centromere to pole movement (anaphase A) was synchronous for all centromeres and occurred coincident with or immediately after spindle pole separation (anaphase B). Chromatin proximal to the centromere is stretched poleward before and during anaphase onset. The stretched chromatin was observed to segregate to the spindle pole bodies at rates greater than centromere to pole movement, indicative of rapid elastic recoil between the chromosome arm and the centromere. These results indicate that the elastic properties of DNA play an as of yet undiscovered role in the poleward movement of chromosome arms.