Genetic profile characterization and population study of 21 autosomal STR in Chinese Kazak ethnic minority group
Genetic profile characterization and population study of 21 autosomal STR in Chinese Kazak ethnic minority group
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中国哈萨克族21个常染色体STR遗传谱特征及群体研究
DOI：
10.1002/elps.201300398
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发表时间：
2014-02-01
期刊：
影响因子：
2.9
通讯作者：
Zhu, Bo-Feng
中科院分区：
文献类型：
--
作者：
Yuan, Jing-Yi;Wang, Xiao-Ye;Zhu, Bo-Feng
短串联重复序列（short tandem repeat，STR）基因座已被公认为法医学应用的重要工具，近几十年来，越来越多新的STR基因座被发现、研究并应用于法医学案件中。本研究调查了哈萨克族21个STR基因座的遗传多态性，以及哈萨克族与其他人群的遗传关系。对新疆伊犁哈萨克自治州114名哈萨克族健康个体的21个STR基因座进行了基因频率分析。我们观察到的159个等位基因的等位基因多样性值范围从0.0044到0.5088。D19 S433位点多态性最高，D1 S1627位点多态性最低。统计分析所产生的数据表明，没有偏离哈迪-温伯格平衡在所有21个STR基因座。为了估计哈萨克族的群体分化，采用分子方差分析的方法，对哈萨克族与邻近群体的所有STR基因座的等位基因频率进行了比较。在2-7个STR基因座上，研究人群与其他人群之间存在显著性差异。根据21个STR基因座的等位基因频率构建邻接树，系统发育分析表明哈萨克族与维吾尔族的亲缘关系较近。本研究结果可为法医学和群体遗传学研究提供有用的信息，也可增加我们对该群体遗传背景的了解。结果表明，21个STR基因座在哈萨克族群体中均具有高度的遗传多态性，为哈萨克族群体遗传信息研究、法医学个体识别和亲权鉴定提供了有价值的群体遗传学资料。
Short tandem repeat loci have been recognized as useful tools in the routine forensic application and in recent decades, more and more new short tandem repeat (STR) loci have been constantly discovered, studied, and applied in forensic caseworks. In this study, we investigated the genetic polymorphisms of 21 STR loci in the Kazak ethnic minority as well as the genetic relationships between the Kazak ethnic minority and other populations. Allelic frequencies of 21 STR loci were obtained from 114 unrelated healthy Kazak individuals in the Ili Kazak Autonomous Prefecture, Xinjiang Uigur Autonomous Region of China. We observed a total of 159 alleles in the group with the allelic diversity values ranging from 0.0044 to 0.5088. The highest polymorphism was found at D19S433 locus and the lowest was found at D1S1627. Statistical analysis of the generated data indicated no deviation from Hardy-Weinberg equilibriums at all 21 STR loci. In order to estimate the population differentiation, allelic frequencies of all STR loci of the Kazak were compared with those of other neighboring populations using analysis of molecular variance method. Statistically significant differences were found between the studied population and other populations at 2-7 STR loci. A neighbor-joining tree was constructed based on allelic frequencies of the 21 STR loci and phylogenetic analysis indicates that the Kazak has a close genetic relationship with the Uigur ethnic group. The present results may provide useful information for forensic sciences and population genetics studies, and can also increase our understanding of the genetic background of this group. The present findings showed that all the 21 STR loci are highly genetically polymorphic in the Kazak group, which provided valuable population genetic data for the genetic information study, forensic human individual identification, and paternity tests.