The oxidative stability of microalgae oil (Schizochytrium aggregatum) and its antioxidant activity after simulated gastrointestinal digestion: Relationship with constituents
The oxidative stability of microalgae oil (Schizochytrium aggregatum) and its antioxidant activity after simulated gastrointestinal digestion: Relationship with constituents
复制标题
DOI：
10.1002/ejlt.201400588
复制
发表时间：
2015-12-01
影响因子：
2.7
通讯作者：
Li, Laihao
中科院分区：
文献类型：
--
作者：
Lv, Junwei;Yang, Xianqing;Li, Laihao
研究了微藻油的化学组成和氧化稳定性，探讨了微藻油经模拟胃肠道消化后的体外生物可利用性和抗氧化活性。通过GC-MS分析，总共鉴定了50多种脂肪酸，其中棕榈酸（38.3%）和DHA（34.5%）被鉴定为主要脂肪酸。用分光光度法测定了各种溶剂提取物中总酚和黄酮的含量，它们的量分别为39.33 ± 0.34 μ g没食子酸/g和16.08 ± 4.3 μ g芦丁/g。HPLC分析表明，β-胡萝卜素、α-生育酚、β-和γ-生育酚（未分离）和δ-生育酚的含量分别为136 μ g/100 g、164.4 μ g/g、317.3 μ g/g和43.2 μ g/g。关于甾醇，胆固醇是4210.5 mg/kg的主要甾醇，其他六种主要甾醇是菜油甾醇（121.4 mg/kg），24-亚甲基胆固醇（192.8 mg/kg），24-甲基-胆甾-7-烯-3 β-醇（144.6 mg/kg）、麦角甾醇（144.8 mg/kg）、豆甾醇（260.1 mg/kg）和Δ 7，24-豆甾二烯醇（150.5 mg/kg）。综合分析表明，微藻油具有良好的油脂品质。采用Schaal烘箱试验评价微藻油的氧化稳定性。采用2，2-二苯基-1-苦肼基（DPPH）自由基清除试验、3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基阳离子脱色活性试验、还原力试验、β-胡萝卜素漂白试验和氧自由基吸收能力（ORAC）抗氧化能力试验，研究了消化油的抗氧化能力。结果表明，微藻油经模拟胃肠道消化后，具有良好的体外生物可利用性和适度的抗氧化能力。因此，微藻油的抗氧化活性主要来自于其丰富的抗氧化成分。
The objective of this study was to examine the chemical composition and oxidative stability of microalgae oil, also to explore in vitro bioaccessibility and antioxidant activity of microalgae oil after simulated gastrointestinal digestion. In total, more than 50 fatty acids were identified by GC-MS analysis, with both palmitic acid (38.3%) and DHA (34.5%) being identified as major fatty acids. The contents of total phenolics and flavonoids in the various solvent extracts were measured spectrometrically, and their amounts were 39.33 +/- 0.34 mu g gallic acid/g and 16.08 +/- 4.3 mu g rutin/g, respectively. HPLC analysis showed that the contents of beta-carotene, alpha-tocopherols, beta- and gamma-tocopherols (not separated) and delta-tocopherols were 136 mu g/100g, 164.4 mu g/g, 317.3 mu g/g, and 43.2 mu g/g, respectively. Concerning sterols, cholesterol was the principal sterol at 4210.5 mg/kg and the other six main sterols were campesterol (121.4 mg/kg), 24-methylene cholesterol (192.8 mg/kg), 24-methyl-colest-7-en-3 beta-ol (144.6 mg/kg), ergosterol (144.8 mg/kg), stigmasterol (260.1 mg/kg) and Delta 7,24-stigmastadienol (150.5 mg/kg), respectively. The overall chemical properties of the tested oils indicated that microalgae oil had a great oil quality. A Schaal oven test was used to evaluate the oxidative stability of microalgae oil. Furthermore, in vitro simulated gastrointestinal digestion was performed, and the antioxidant ability of digestion oil was determined by using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, a 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical cation decolourisation activity assay, a reducing power assay, a beta-carotene bleaching assay and an oxygen radical absorbance capacity (ORAC) antioxidant assay. The results showed that following simulated gastrointestinal digestion, microalgae oil displayed a good in vitro bioaccessibility and moderate antioxidant capacity. Thus, the antioxidant activity of the microalgae oil was mainly contributed by its abundant antioxidant constituents.