Ultrasound enhanced drug toxicity on Chinese hamster ovary cells in vitro.
Ultrasound enhanced drug toxicity on Chinese hamster ovary cells in vitro.
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DOI：
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发表时间：
1989-11
期刊：
影响因子：
11.2
通讯作者：
A. H. Saad;G. Hahn
中科院分区：
文献类型：
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作者：
A. H. Saad;G. Hahn
将中国仓鼠卵巢细胞（HA 1）在37-43 ℃的温度下暴露于存在各种药物的治疗超声（F = 2.025 MHz）。使用的空间平均强度为0.5-2 W/cm 2。随后使用克隆形成试验测试这些细胞的存活。超声可明显增强阿霉素和阿霉素B的细胞毒作用。对于阿霉素，增强作用取决于超声处理的强度（暴露持续时间为30分钟）。在0.5 W/cm 2时，在41 ℃以上细胞毒性增强。在1 W/cm 2时，37 ℃下的细胞毒性增加了3个数量级。因此，强度的增加导致“阈值”温度的降低。阿霉素的作用部分可以通过细胞对药物的净吸收增加来解释。此外，观察到超声波增加细胞对阿霉素的敏感性。对于黄曲霉素B，仅在暴露持续时间大于30分钟和43 ℃时观察到增强。顺铂和依托泊苷没有观察到增强作用。从这些结果来看，似乎超声增强了药物的细胞毒性，其作用模式（至少部分）涉及质膜。
Chinese hamster ovary cells (HA1) were exposed to therapeutic ultrasound (F = 2.025 MHz) in the presence of various drugs at temperatures of 37-43 degrees C. The space averaged intensities used were 0.5-2 W/cm2. The survival of these cells was subsequently tested using the clonogenic assay. Marked enhancement by ultrasound of the cytotoxicity of Adriamycin and amphotericin B was observed. For Adriamycin, the potentiation was dependent upon the intensity of sonication (exposure duration being 30 min). At 0.5 W/cm2, there was enhancement of cytotoxicity above 41 degrees C. At 1 W/cm2, there was a 3-order increase in cytotoxicity at 37 degrees C. Thus an increase in intensity resulted in a decrease in "threshold" temperature. The effect with Adriamycin could be explained in part by an increase in net uptake of drug into the cells. Further, ultrasound was observed to increase the sensitivity of cells to Adriamycin. For amphotericin B, the enhancement was observed only at exposure durations greater than 30 min and at 43 degrees C. There was no enhancement observed for cisplatin and etoposide. From these results, it appears that ultrasound potentiates the cytotoxicity of drugs the mode of action of which (at least in part) involves the plasma membrane.