Pectic Polysaccharides from Panax ginseng as the Antirotavirus Principals in Ginseng
Pectic Polysaccharides from Panax ginseng as the Antirotavirus Principals in Ginseng
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DOI：
10.1021/bm100397p
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发表时间：
2010-08-01
影响因子：
6.2
通讯作者：
Park, Jeong Hill
中科院分区：
文献类型：
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作者：
Baek, Seung-Hoon;Lee, Jin Gyun;Park, Jeong Hill
为了评价人参的止泻作用，在轮状病毒感染的体外模型上对人参的有效成分进行了研究。从人参热水提取物中分离纯化出两种果胶多糖，分别命名为GP50-DHR(56.0 kDa)和GP50-EHR(77.0 kDa)。两种多糖均能剂量依赖性地挽救轮状病毒感染的细胞活力(IC50分别为15和10微克/毫升)。这两种多糖都具有鼠李糖醛酸I型毛状区的高半乳糖醛酸骨架的共同结构特征，具有丰富的支化点的富含阿拉伯糖的侧链是GP50-EHR所独有的，可能有助于GP50-EHR比GP50-DHR具有更强的抗轮状病毒作用。由于高半乳糖醛酸本身没有显示出抗轮状病毒的作用，毛状区可能是功能部位。值得注意的是，这两种多糖的抗轮状病毒作用是由于抑制了轮状病毒与细胞的附着。结合广泛的非细胞毒性，这些发现表明人参多糖是治疗轮状病毒腹泻的可行选择。
To evaluate the antidiarrheal effect of ginseng, the active principals of ginseng were studied in vitro model of rotavirus infection, the leading cause of severe diarrhea. Two pectic polysaccharides, named as GP50-dHR (56.0 kDa) and GP50-eHR (77.0 kDa), were purified from hot water extract of ginseng by bioassay-linked fractionation. Both polysaccharides rescued cell viability from rotavirus infection dose-dependently (IC50 are 15 and 10 mu g/mL, respectively). Both polysaccharides had common structural features of homogalacturonan backbone with hairy regions of rhamnogalacturonan type I. Arabinose-rich side chains with abundant branch points were unique in GP50-eHR and may contribute to a greater antirotavirus effect of GP50-eHR than GP50-dHR. Because homogalacturonan itself did not show an antirotavirus effect, hairy regions might be functional sites. Of note, the antirotavirus effect of both polysaccharides resulted from inhibiting rotavirus attachment to cells. Together with a wide range of noncytotoxicity, these findings suggest that ginseng polysaccharides are viable therapeutic options for rotavirus diarrhea.