Coupling frontal elution paper chromatography with desorption corona beam ionization mass spectrometry for rapid analysis of chlorphenamine in herbal medicines and dietary supplements
Coupling frontal elution paper chromatography with desorption corona beam ionization mass spectrometry for rapid analysis of chlorphenamine in herbal medicines and dietary supplements
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正面洗脱纸色谱法与解吸电晕束电离质谱联用快速分析草药和膳食补充剂中的扑尔敏
DOI：
10.1016/j.chroma.2011.08.067
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发表时间：
2011-10-14
影响因子：
4.1
通讯作者：
Feng, Yu-Qi
中科院分区：
文献类型：
--
作者：
Huang, Yun-Qing;You, Jing-Qing;Feng, Yu-Qi
建立了一种将前沿洗脱纸色谱与解吸电晕束离子化质谱（DCBI-MS）联用的快速检测中草药或保健品中氯苯那敏的方法。在该方法中，将草药产品的乙醇提取物直接点样到等腰三角形滤纸片上，然后在强洗脱条件下展开滤纸片，样品区在溶剂前沿迁移。分析物最终在V形尖端处冷凝，然后可以将V形尖端置于DCBI的可见等离子体束下进行电离。整个过程花了不到5分钟。在这项工作中使用的三角形板上的正面洗脱纸色谱提高了氯苯那敏的信号强度的30倍，由于在尖端的分析物冷凝和减少的背景抑制。此外，在固体或粉末样品的分析中，纸张还起到过滤器的作用，这可以通过省略离心步骤来增加分析通量。该方法已成功地应用于中草药中扑尔敏的测定。在本研究中检查的12种草药中，有4种检测到氯苯那敏。在全扫描正离子模式下，检测限为200 ng/mL（绝对值为2.0 ng），线性范围为5.01 μ g/mL ~ 50 μ g/mL，相关系数R（2）= 0.895。氯苯那敏的回收率为84.3 ~ 90.6%，相对标准偏差（RSD）小于15.0%。(C)2011 Elsevier B. V.保留所有权利。
We developed a convenient method by coupling frontal elution paper chromatography with desorption corona beam ionization mass spectrometry (DCBI-MS) for rapid determination of chlorphenamine added in herbal medicines or dietary supplements. In this method, the ethanol extract of the herbal products was spotted directly onto an isosceles triangular filter paper sheet, and then the paper sheet was developed under strong elution condition with the sample zone migrating at the solvent front. The analyte was finally condensed at the V-shaped tip which could then be placed under the visible plasma beam of DCBI for ionization. The overall procedure took less than 5 min. The frontal elution paper chromatography on a triangular plate used in this work improved the signal intensity of chlorphenamine by 30-fold due to the analyte condensing at the tip and the reduction of the background suppression. Furthermore, the paper sheet also functioned as a filter in the analysis of solid or powder samples, which can increase the analytical throughput by omitting the step of centrifugation. The proposed method in current study was successfully applied in the determination of chlorphenamine in herbal medicines. Chlorphenamine was detected in four of the twelve types of herbal medicines examined in this study. The limit of detection was 200 ng/mL (2.0 ng absolute) in full-scan positive-ion mode and the linear range was from 5.01 mu g/mL to 50 mu g/mL with satisfactory linear coefficient (R(2) (the square of the correlation coefficient) = 0.895). Good reproducibility was achieved with relative standard deviations (RSDs) less than 15.0% and the recoveries of chlorphenamine ranged from 84.3 to 90.6%. (C) 2011 Elsevier B.V. All rights reserved.