Lens-free shadow image based high-throughput continuous cell monitoring technique
Lens-free shadow image based high-throughput continuous cell monitoring technique
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DOI：
10.1016/j.bios.2012.05.022
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发表时间：
2012-10-01
影响因子：
12.6
通讯作者：
Seo, Sungkyu
中科院分区：
文献类型：
--
作者：
Jin, Geonsoo;Yoo, In-Hwa;Seo, Sungkyu
证明了不需要任何标记试剂或样品破坏的高通量连续细胞监测技术。在成本和空间有效的无镜头阴影成像平台内，与单个数字图像同时且连续地监测了6000多个人肺泡上皮A549细胞。在与无镜头阴影成像平台集成的自定义构建孵化器中进行的实验中，可以通过计算信噪比（SNRS）和细胞的阴影直径（SDS）来成功表征细胞核分裂过程。阴影模式。该平台的多功能性还启用了单个细胞活力测试，然后进行了实时细胞计数。这项研究首先表明，无镜头的阴影成像技术可以提供连续的细胞监测，而无需任何染色/标记试剂和样品破坏。这种基于无镜头阴影成像的高通量连续细胞监测技术可广泛用作药物和食物筛查或细胞增殖和生存能力测试领域的紧凑，低成本和高通量细胞监测工具。 （c）2012 Elsevier B.V.保留所有权利。
A high-throughput continuous cell monitoring technique which does not require any labeling reagents or destruction of the specimen is demonstrated. More than 6000 human alveolar epithelial A549 cells are monitored for up to 72 h simultaneously and continuously with a single digital image within a cost and space effective lens-free shadow imaging platform. In an experiment performed within a custom built incubator integrated with the lens-free shadow imaging platform, the cell nucleus division process could be successfully characterized by calculating the signal-to-noise ratios (SNRs) and the shadow diameters (SDs) of the cell shadow patterns. The versatile nature of this platform also enabled a single cell viability test followed by live cell counting. This study firstly shows that the lens-free shadow imaging technique can provide a continuous cell monitoring without any staining/labeling reagent and destruction of the specimen. This high-throughput continuous cell monitoring technique based on lens-free shadow imaging may be widely utilized as a compact, low-cost, and high-throughput cell monitoring tool in the fields of drug and food screening or cell proliferation and viability testing. (C) 2012 Elsevier B.V. All rights reserved.