In vivo gene transfer into the mouse uterus: A powerful tool for investigating implantation physiology
In vivo gene transfer into the mouse uterus: A powerful tool for investigating implantation physiology
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DOI：
10.1016/j.jri.2005.06.006
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发表时间：
2005-10-01
影响因子：
3.4
通讯作者：
Murata, Y
中科院分区：
文献类型：
--
作者：
Kimura, T;Nakamura, H;Murata, Y
着床期子宫内膜中瞬时基因的体内转移为从分子水平分析着床期的生理/病理生理学提供了很好的机会。我们在这里回顾了为此目的而应用的方法。病毒载体被广泛用于体内基因治疗模型，然而，使用这种载体将基因转移到子宫中还没有成功的例子。基于阳离子脂质体的技术已经取得了一些成功的结果，导致了植入生理的改变。应用日本包膜血凝病毒(HVJ-E)载体系统，转染率远高于以阳离子脂质体为基础的方法。商业HVJ-E载体(GenomONE-Neo(TM))现已面市。几个成功的体内基因转移的例子表明，降钙素、Hoxa 10和核因子kappaB在决定着床效率或时机方面发挥着重要作用。在此基础上，我们应该进一步分析人类材料植入失败的病理生理学。(C)2005爱思唯尔爱尔兰有限公司。保留所有权利。
In vivo transient transfection of cDNA into uterine endometrium during the implantation period provides great opportunities to analyse the physiology/pathophysiology of implantation at the molecular level. We review here methodologies which have been applied for this purpose. Viral vectors are widely used for in vivo gene therapy models; however, there is no successful example of gene transfer into the uterus using such vectors. Cationic liposome-based technologies have produced some successful results, causing alterations in implantation physiology. We applied a haernagglutinating virus of Japan envelope (HVJ-E) vector system and showed that the transfection efficiency was much higher than that of methods based on cationic liposome. Commercial HVJ-E vector (GenomONE-Neo (TM)) is now also available. Several successful examples of in vivo gene transfer revealed that calcitonin, Hoxa 10 and NF kappaB play important roles in determining the efficiency or timing of implantation. Based on this knowledge, we should further analyse the pathophysiology of human implantation failure using human materials. (c) 2005 Elsevier Ireland Ltd. All rights reserved.