Microvasculature and VEGF expression in cartilaginous tumors
Microvasculature and VEGF expression in cartilaginous tumors
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DOI：
10.1016/s0046-8177(00)80248-8
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发表时间：
2000-03-01
期刊：
影响因子：
3.3
通讯作者：
Lackman, R
中科院分区：
文献类型：
--
作者：
Ayala, G;Liu, C;Lackman, R
本文对26例软骨病变(包括5例内生性软骨瘤、9例1级软骨肉瘤、6例2级软骨肉瘤、4例3级软骨肉瘤、1例间叶性软骨肉瘤和1例粘液样软骨肉瘤)的微血管和血管内皮生长因子的表达进行了检测。用HE切片和因子相关抗原免疫组织化学染色计数微血管数来衡量新生血管的程度。血管分为支架周围血管(PCV)和支架内血管(ICV)。PVC包括小叶周围或侵袭小叶的血管，但本身被非软骨性间质(即纤维间质)包围；ICV由肿瘤结节内与恶性细胞或肿瘤间质直接接触的血管组成。CS和颈动脉周围血管的组织学类型和分级呈正相关。相反，ICV仅见于较高级别的CS，无内生软骨瘤，仅1/9的1级CS有ICV。这位病人患的是油性疾病。2级CS中，除2例外，其余均显示ICV(平均20.5)。例外主要是1级CS伴2级局灶性区域和广泛的坏死区。除1例3级CS外，除1例CS转移至肺外，其余均有ICV。高级别病变的恶性软骨细胞强烈表达血管内皮生长因子(VEGF)，这是一种强有力的血管生成因子。唯一未表达血管内皮生长因子的高级别肿瘤也未显示ICV。内生性软骨瘤和1级CS，多数无ICV，均不表达VEGF。综上所述，PCV存在于所有类型的肿瘤软骨中，且数量随组织学分级增加而增加；ICV可见于高级别病变，例外表现为广泛的坏死；恶性软骨细胞表达的血管内皮生长因子几乎仅见于高级别病变，其中显示椎管内血管的病变。PCV可能参与支持肿瘤的生长，而ICV可能参与软骨肿瘤转移潜能的获得。血管内皮生长因子的表达与ICV的存在密切相关。版权所有(C)2000，由W.B.Saunders公司提供。
We examined the microvasculature and VEGF expression in 26 cartilaginous lesions (CL) including 5 enchondromas, 9 grade 1 chondrosarcoma (CS), 6 grade 2 CS, 4 grade 3 CS, 1 mesenchymal, and 1 myxoid chondrosarcoma. The degree of neovascularization was measured by counting microvessels on H&E and factor VIII related antigen immunostained slides. Vessels were divided into pericartilage vessels (PCV) and intracartilage vessels (ICV). PVC comprised vessels around the lobules or invading the lobules but themselves surrounded by noncartilaginous stroma (ie, fibrous stroma); ICV consisted of those vessels present inside the tumoral nodules and in direct apposition with malignant cells or tumoral stroma. A direct correlation was seen between histological type and grade of CS and pericartilage vessels. In contrast, ICV were found only in higher-grade CS, No enchondromas and only 1 of 9 grade 1 CS had ICV. This patient had Oilier's disease. All but 2 of the grade 2 CS showed ICV (average, 20.5). The exceptions were predominantly grade 1 CS with focal grade 2 areas and extensive areas of necrosis. All but 1 grade 3 CS had ICV, the exception being a case of metastatic CS to the lung. Malignant chondrocytes of high-grade lesions stained strongly for vascular endothelial growth factor (VEGF), a potent angiogenic factor. The only high-grade tumors that did not express VEGF did not show ICV either. Enchondromas and grade 1 CS, most without ICV, did not express VEGF. In summary, PCV are present in all categories of tumoral cartilage and the number increases with histological grade; ICV are found in high-grade lesions, and the exceptions show extensive necrosis; VEGF expression by malignant chondrocytes is seen in high-grade lesions almost exclusively and among these in those lesions that showed intracartilage vessels. It is possible that PCV are involved in supporting tumor growth, whereas ICV might be involved in the acquisition of metastatic potential by cartilage tumors. VEGF expression is strongly associated with the presence of ICV. Copyright (C) 2000 by W.B. Saunders Company.