Can Reinforcement of Maxillary Implant Overdentures Decrease the Stress on Underlying Implants?
Can Reinforcement of Maxillary Implant Overdentures Decrease the Stress on Underlying Implants?
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上颌种植体覆盖义齿的加固能否减少下方种植体的应力？
DOI：
10.11607/jomi.4921
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Maeda Y.
中科院分区：
文献类型：
--
作者：
Takahashi T;Gonda T;Maeda Y.
目的：评价一种研究小型猪种植体周围早期骨形成的新方法。除了传统的组织学和组织形态计量学分析外，还从信使核糖核酸和蛋白质水平分析了成骨基因的表达。材料和方法：在6只小型猪上拔除下颌前磨牙和第一磨牙。愈合3个月后，植入了36个特别设计的骨腔内植入物。使用了三种不同的种植体表面配置：钛SLA、钛SLActive和钛锆SLActive(Roxolid)。每个半下颌骨在牙弓两侧随机分配三个种植体(每种表面类型一个)，采用开口设计。三只动物在种植后3天后处死，另3只在种植后愈合2周后处死。对每只动物的右半下颌骨进行定性组织学和定量组织形态计量学分析。取左半下颌骨进行免疫组织化学荧光(IHF)分析。β-连环蛋白、Runx2、骨桥蛋白和骨钙素的检测采用半定量逆转录聚合酶链式反应(QRT-PCR法)。结果：种植后3d，所有种植体均被血栓包围。在房间内没有观察到临时基质或骨。无感染或退行性病变。2周时，组织形态计量学分析显示，两组间的骨面积差异无统计学意义。QRT-PCR结果显示，TiSLActive的骨钙素基因表达最高，其次是TiSLA和Roxolid SLActive。TiSLA和Roxolid SLActive的Osterix mRNA表达高于TiSLActive。这些差异在统计学上并不显著。IHF仅在2周时骨钙素呈阳性。3天时，仅在天然骨中检测到骨钙素。2周时，TiSLActive组骨钙素表达最高，Roxolid SLActive组次之，TiSLA组次之，但组间骨钙素表达水平差异无统计学意义。结论：目前的方法可以评估成骨早期基因表达的变化，这些变化似乎与表面的质量有关。需要用更高的能力和更特异的抗体进行进一步的研究来证实这些初步发现。
Purpose: A novel approach for the study of early bone formation around dental implants in the miniature pig was evaluated. In addition to the traditional histologic and histomorphometric analysis, the expression of the osteogenic genes was analyzed both at the messenger ribonucleic acid (mRNA) and protein level. Materials and Methods: Mandibular premolars and the first molar were extracted in six miniature pigs. After 3 months of healing, 36 specially designed bone chamber implants were placed. Three different implant surface configurations were used: titanium SLA, titanium SLActive, and titanium zirconium SLActive (Roxolid). Each hemi-mandible received three randomly allocated implants (one for each surface type) on both sides of the arch, in a split-mouth design. Three animals were sacrificed after 3 days and another three after 2 weeks of healing post–implant insertion. For each animal the right hemi-mandible underwent qualitative histologic and quantitative histomorphometric analysis. The left hemi-mandible underwent immunohistofluorescence (IHF) analysis. β-catenin, Runx2, osteopontin, and osteocalcin were analyzed by IHF; osterix, and osteocalcin mRNA expression was also evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results: At 3 days after the implantation, all implants were surrounded by blood clot. No provisional matrix or bone was observed inside the chamber. Infection or degenerative lesions were absent. At 2 weeks, the histomorphometric analysis showed no significant difference between the groups concerning the bone area. qRT-PCR showed that Ti SLActive had the highest osteocalcin mRNA expression followed by Ti SLA and Roxolid SLActive. Osterix mRNA expression was higher on Ti SLA and Roxolid SLActive compared to Ti SLActive. The differences were not statistically significant. IHF was only found positive for osteocalcin at 2 weeks. At 3 days, osteocalcin was detected only on native bone. At 2 weeks, osteocalcin was expressed highest by Ti SLActive followed by Roxolid SLActive and TiSLA; however, there was no statistically significant difference between the groups in the osteocalcin expression level. Conclusion: The present methodology allowed evaluation of changes in gene expression during the early phase of osteogenesis that seem to be related to the quality of the surface. Further studies with higher power and more specific antibodies are needed to confirm these preliminary findings.