Proteins of Diverse Function and Subcellular Location Are Lysine Acetylated in Arabidopsis
Proteins of Diverse Function and Subcellular Location Are Lysine Acetylated in Arabidopsis
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DOI：
10.1104/pp.110.171595
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发表时间：
2011-04-01
期刊：
影响因子：
7.4
通讯作者：
Sweetlove, Lee J.
中科院分区：
文献类型：
--
作者：
Finkemeier, Iris;Laxa, Miriam;Sweetlove, Lee J.
赖氨酸（Lys）的ε -氨基乙酰化是一种可逆的翻译后修饰，最近发现在哺乳动物细胞的大多数亚细胞位置，发生在细胞核外的蛋白质上。除了对细胞核组蛋白乙酰化的充分研究外，对植物中的这种修饰几乎一无所知。在这里，我们报道了植物中的赖氨酸乙酰化也发生在细胞器和细胞质蛋白上。我们在74种不同功能类别的蛋白质上鉴定了91个赖氨酸乙酰化位点。此外，我们的研究表明，Lys乙酰化可能是叶绿体中一个重要的翻译后修饰，因为四个卡尔文循环酶被乙酰化。Rubisco的质体编码的大亚基之所以突出，是因为大量的乙酰化位点发生在参与Rubisco三级结构形成和催化功能的重要赖氨酸残基上。利用重组人去乙酰化酶sirtuin 3，证明了Lys去乙酰化显著影响Rubisco活性以及其他中心代谢酶的活性，如卡尔文循环酶磷酸甘油酸激酶、糖酵解酶甘油醛3-磷酸脱氢酶和三羧酸循环酶苹果酸脱氢酶。我们的研究结果表明，在拟南芥（Arabidopsis thaliana）中，赖氨酸乙酰化也发生在细胞核外的蛋白质上，并且赖氨酸乙酰化可能在关键代谢酶的调节中起重要作用。
Acetylation of the epsilon-amino group of lysine (Lys) is a reversible posttranslational modification recently discovered to be widespread, occurring on proteins outside the nucleus, in most subcellular locations in mammalian cells. Almost nothing is known about this modification in plants beyond the well-studied acetylation of histone proteins in the nucleus. Here, we report that Lys acetylation in plants also occurs on organellar and cytosolic proteins. We identified 91 Lys-acetylated sites on 74 proteins of diverse functional classes. Furthermore, our study suggests that Lys acetylation may be an important posttranslational modification in the chloroplast, since four Calvin cycle enzymes were acetylated. The plastid-encoded large subunit of Rubisco stands out because of the large number of acetylated sites occurring at important Lys residues that are involved in Rubisco tertiary structure formation and catalytic function. Using the human recombinant deacetylase sirtuin 3, it was demonstrated that Lys deacetylation significantly affects Rubisco activity as well as the activities of other central metabolic enzymes, such as the Calvin cycle enzyme phosphoglycerate kinase, the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase, and the tricarboxylic acid cycle enzyme malate dehydrogenase. Our results demonstrate that Lys acetylation also occurs on proteins outside the nucleus in Arabidopsis (Arabidopsis thaliana) and that Lys acetylation could be important in the regulation of key metabolic enzymes.