Cloning and expression pattern of gsdf during the first maleness reproductive phase in the protandrous Acanthopagruss latus
Cloning and expression pattern of gsdf during the first maleness reproductive phase in the protandrous Acanthopagruss latus
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早熟刺鲃第一个雄性生殖阶段gsdf的克隆和表达模式
DOI：
10.1016/j.ygcen.2015.02.018
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发表时间：
2015-06-01
影响因子：
2.7
通讯作者：
Chen, Shi Xi
中科院分区：
文献类型：
--
作者：
Chen, Yuan;Hong, Wan Shu;Chen, Shi Xi
性腺体细胞衍生因子（Gsdf）是转化生长因子β超家族的新成员。作为硬骨鱼和性腺特异性生长因子，多项研究表明 Gsdf 在早期生殖细胞发育中发挥着重要作用。在本研究中，首次从早熟物种棘鲃（Acanthopagrus latus）中克隆了一个 1700 bp 长的 gsdf 基因。我们进一步分析了第一个雄性生殖阶段 gsdf 在各自睾丸和卵巢区域的细胞定位和表达模式。结果表明，在性别分化过程中，gsdf转录本在卵睾中高表达，并且睾丸区体细胞表达的gsdf转录本远多于卵巢区体细胞。青春期开始时，gsdf 表达水平在精子发生过程中逐渐下降。相反，卵巢区表现出稳定的增加模式，与血浆 17 β-雌二醇 (E-2) 水平相似。这些结果表明 Gsdf 可能参与早期生殖细胞发育，例如生殖细胞发育。 A. latus 精原细胞和卵原细胞的增殖和分化。 (C) 2015 Elsevier Inc. 保留所有权利。
Gonadal soma-derived factor (Gsdf) is a new member of the transforming growth factor beta superfamily. As a teleost- and gonad-specific growth factor, several studies indicate that Gsdf plays an important role in early germ cell development. In the present study, for the first time, a 1700-bp long gsdf gene was cloned from a protandrous species, Acanthopagrus latus. We further analyzed the cellular localization and the expression patterns of gsdf in respective testicular and ovarian zones during the first maleness reproductive phase. The results showed that gsdf transcripts were highly expressed in the ovotestis during sex differentiation, and the somatic cells of the testicular zone expressed many more gsdf transcripts than those of the ovarian zone. At the onset of puberty, the gsdf expression levels decreased gradually during spermatogenesis. Conversely, the ovarian zone exhibited a stable increase pattern which was similar to the plasma 17 beta-estradiol (E-2) levels. These results suggested that Gsdf may participates in early germ cell development, e.g. proliferation and differentiation of spermatogonia and oogonia in A. latus. (C) 2015 Elsevier Inc. All rights reserved.