Effect of Storage of Spawn on Mycelial Growth, Fruit-Body Formation and Mycelial Constituents of the Shiitake Mushroom (Lentinus edodes)
Effect of Storage of Spawn on Mycelial Growth, Fruit-Body Formation and Mycelial Constituents of the Shiitake Mushroom (Lentinus edodes)
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菌种贮藏对香菇菌丝生长、子实体形成及菌丝成分的影响
DOI：
10.2525/ecb1963.35.283
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
J. Shishiyama
中科院分区：
文献类型：
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作者：
T. Terashita;Saeko Hirata;K. Yoshikawa;J. Shishiyama
研究了短时间（15 ~ 200 d）冷藏和常温储菌对3株香菇菌丝生长、子实体产量、菌丝化学成分和酶活性的影响。在4℃、15℃和24℃条件下分别保存200天、160天和60天，菌丝生长和子实体产量均无显著下降。菌丝生长在4°C或15°C条件下贮存60-110天后有所增加。在所有测试菌株中都观察到这些影响。在15℃条件下贮藏30 d，子实体产量增加。然而，在低温（4°C, 15°C）下较长时间（160-200天）的储存会减少形态正常的子实体的形成。相比之下，与未储存的卵（对照）相比，在24°C下储存较短时间后，正常的子实体形成增加。贮藏菌种孵育后菌丝中总糖含量显著降低，而蛋白质和游离氨基酸含量无明显下降。贮藏菌种孵育后菌丝蛋白酶活性无显著差异。淀粉酶、β- 1,3 -葡聚糖酶和海藻化酶活性随卵的存放而显著降低。
The effects of short periods (15-200 days) of either refrigerated or room temperature spawn storage on the mycelial growth, fruit-body yield, chemical constituents and enzyme activities of the mycelia of three strains of Lentinus edodes were investigated. No significant decrease in the mycelial growth and fruit-body yield was observed under storage for 200 days at 4°C, 160 days at 15°C or 60 days at 24°C. Mycelial growth increased upon spawn storage for 60-110 days at 4°C or 15°C. These effects were observed in all strains tested. The fruit-body yield increased on storing the spawn for 30 days at 15°C. However, storage of the spawn for relatively longer periods (160-200 days) at low temperature (4°C, 15°C) decreased the formation of morphologically normal fruit-bodies. In contrast, normal fruit-body formation increased upon storage for shorter periods at 24°C compared with the not-stored spawn (control) . Total sugar in mycelia after incubating the stored spawn decreased significantly, whereas the protein and free amino acid content did not show any decrease. Proteinase activity in mycelia after incubating the stored spawn did not differ significantly. However, amylase, β-1, 3-glucanase and trehalase activity decreased markedly upon storage of the spawn.