Differences in the localization and morphology of chromosomes in the human nucleus.
Differences in the localization and morphology of chromosomes in the human nucleus.
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DOI：
10.1083/jcb.145.6.1119
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发表时间：
1999-06-14
期刊：
影响因子：
--
通讯作者：
Bickmore WA
中科院分区：
文献类型：
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作者：
Croft JA;Bridger JM;Boyle S;Perry P;Teague P;Bickmore WA
通过荧光原位杂交，我们发现人类18号和19号染色体在核位置、染色体形态以及与核亚结构的相互作用方面存在显著差异。结果显示，人类19号染色体在细胞核中所处的位置比18号染色体更靠内，并且与核基质的结合更为广泛。18号染色体更偏向核周边的定位在细胞周期早期就已确定，并在此后得以维持。我们发现，18号和19号染色体在细胞核中的优先定位反映在易位染色体在细胞核中的方向上。最后，我们表明转录抑制可对染色体结构产生显著但可逆的影响。我们的数据表明，染色体之间基因组序列的分布对核结构有影响，并且我们结合一个具有功能分区的人类细胞核模型对我们的研究结果进行了讨论。
Using fluorescence in situ hybridization we show striking differences in nuclear position, chromosome morphology, and interactions with nuclear substructure for human chromosomes 18 and 19. Human chromosome 19 is shown to adopt a more internal position in the nucleus than chromosome 18 and to be more extensively associated with the nuclear matrix. The more peripheral localization of chromosome 18 is established early in the cell cycle and is maintained thereafter. We show that the preferential localization of chromosomes 18 and 19 in the nucleus is reflected in the orientation of translocation chromosomes in the nucleus. Lastly, we show that the inhibition of transcription can have gross, but reversible, effects on chromosome architecture. Our data demonstrate that the distribution of genomic sequences between chromosomes has implications for nuclear structure and we discuss our findings in relation to a model of the human nucleus that is functionally compartmentalized.