Determination of the estrous cycle phases of rats: some helpful considerations
Determination of the estrous cycle phases of rats: some helpful considerations
复制标题
DOI：
10.1590/s1519-69842002000400008
复制
发表时间：
2002-11-01
影响因子：
--
通讯作者：
TANNO, A. P.
中科院分区：
文献类型：
--
作者：
MARCONDES, F. K.;BIANCHI, F. J.;TANNO, A. P.
大鼠的发情周期较短，这使它们成为研究生殖周期变化的理想选择。发情周期为4天，特征为：发情前期、发情期、发情期、退情期，可根据阴道涂片中观察到的细胞类型来确定。由于阴道分泌物的收集和染色材料的使用通常需要一些时间，因此本研究的目的是为研究人员提供一些有用的考虑，以快速实用的方式确定大鼠的发情周期阶段。在一个月的时间里，每天早晨采集30只雌性大鼠阴道分泌物，不借助聚光镜，在显微镜下观察未染色的天然物质。使用10倍物镜，更容易分析阴道涂片中存在的三种细胞类型之间的比例。使用40X物镜，更容易识别每一种细胞类型。30只雌性大鼠采集阴道灌洗液，在显微镜下观察材料，并测定发情周期，时间均为15-20分钟。
The short length of the estrous cycle of rats makes them ideal for investigation of changes occurring during the reproductive cycle. The estrous cycle lasts four days and is characterized as: proestrus, estrus, metestrus and diestrus, which may be determined according to the cell types observed in the vaginal smear. Since the collection of vaginal secretion and the use of stained material generally takes some time, the aim of the present work was to provide researchers with some helpful considerations about the determination of the rat estrous cycle phases in a fast and practical way. Vaginal secretion of thirty female rats was collected every morning during a month and unstained native material was observed using the microscope without the aid of the condenser lens. Using the 10X objective lens, it was easier to analyze the proportion among the three cellular types, which are present in the vaginal smear. Using the 40X objective lens, it is easier to recognize each one of these cellular types. The collection of vaginal lavage from the animals, the observation of the material, in the microscope, and the determination of the estrous cycle phase of all the thirty female rats took 15-20 minutes.