A developmental switch of AMPA receptor subunits in neocortical pyramidal neurons
A developmental switch of AMPA receptor subunits in neocortical pyramidal neurons
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DOI：
10.1523/jneurosci.22-08-03005.2002
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发表时间：
2002-04-15
影响因子：
5.3
通讯作者：
Huguenard, JR
中科院分区：
文献类型：
--
作者：
Kumar, SS;Bacci, A;Huguenard, JR
AMPA受体介导了大脑中大部分的快速兴奋性神经传递，而那些缺乏谷氨酸受体2（GluR 2）亚基的受体是Ca 2+渗透性的，主要通过抑制性中间神经元在皮质结构中表达。在这里，我们报告说，突触AMPA受体兴奋层5锥体神经元在大鼠新皮层缺乏GluR 2在早期发展，大约出生后第16天之前，证明了他们的内向整流电流-电压关系，AMPA受体介导的EPSCs的外部和内部的多胺，Ca 2+的渗透性，和GluR 2免疫反应。总体而言，这些结果表明，新皮层锥体神经元通过改变其亚基组成，在其AMPA受体的Ca 2+渗透性中经历发育开关。这对可塑性和神经毒性具有重要意义。
AMPA receptors mediate most of the fast excitatory neurotransmission in the brain, and those lacking the glutamate receptor 2 (GluR2) subunit are Ca2+-permeable and expressed in cortical structures primarily by inhibitory interneurons. Here we report that synaptic AMPA receptors of excitatory layer 5 pyramidal neurons in the rat neocortex are deficient in GluR2 in early development, approximately before postnatal day 16, as evidenced by their inwardly rectifying current-voltage relationship, blockade of AMPA receptor-mediated EPSCs by external and internal polyamines, permeability to Ca2+, and GluR2 immunoreactivity. Overall, these results indicate that neocortical pyramidal neurons undergo a developmental switch in the Ca2+ permeability of their AMPA receptors through an alteration of their subunit composition. This has important implications for plasticity and neurotoxicity.