Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model.
Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model.
复制标题
DOI：
10.1155/2016/5876836
复制
发表时间：
2016
影响因子：
4.3
通讯作者：
Savitz SI
中科院分区：
文献类型：
--
作者：
Yang B;Parsha K;Schaar K;Satani N;Xi X;Aronowski J;Savitz SI
自体骨髓（BM）来源的单核细胞（MNC）的全身给药正在被研究作为治疗缺血性中风的一种新的治疗方式。自体应用增加了跨国公司可能被存储为银行来源的可能性。目前还没有研究探讨低温保存 BM-MNCs对其在中风模型中的功能的影响。在本研究中，C57BL/6 小鼠接受大脑中动脉闭塞 (MCAo) 60 分钟，然后分为两个治疗组：新鲜 MNC 与冷冻保存的 MNC。 MCAo后22小时收集BM-MNCs，并在冷冻保存的MNCs组中在液氮中保存12个月。通过流式细胞术评估小鼠 BM-MNC 各个亚群的 BM-MNC 细胞活力、组成和表型，并通过角试验和阶梯梯级试验，用新鲜收获的 MNC 与冷冻保存的 MNC 测试中风动物的行为恢复情况。我们发现长期冷冻保存会对骨髓跨国公司的细胞活力产生负面影响。冷冻保存还会改变跨国公司内各种亚群的细胞组成。然而，尽管在冷冻保存的细胞中观察到了这些变化，但新鲜和冷冻的 MNC 对行为和组织学结果具有相似的有益作用。
The systemic administration of autologous bone marrow (BM) derived mononuclear cells (MNCs) is under investigation as a novel therapeutic modality for the treatment of ischemic stroke. Autologous applications raise the possibility that MNCs could potentially be stored as a banked source. There have been no studies that investigate the effects of cryopreservation of BM-MNCs on their functional abilities in stroke models. In the present study, C57BL/6 mice were subjected to middle cerebral artery occlusion (MCAo) for 60 minutes and then divided into two treatment groups: fresh MNCs versus cryopreserved MNCs. BM-MNCs were collected at 22 hours after MCAo and were stored in liquid nitrogen for 12 months in cryopreserved MNCs group. BM-MNCs cellular viability, composition, and phenotype of the various subpopulations of mice BM-MNCs were evaluated by flow cytometry, and the behavioral recovery of stroke animals was tested with freshly harvested MNCs versus cryopreserved MNCs by corner test and ladder rung test. We found that long-term cryopreservation negatively impacts the cellular viability of bone marrow MNCs. Cryopreservation also alters the cellular composition of various subpopulations within the MNCs. However, despite the changes observed in cryopreserved cells, both fresh and frozen MNCs have similar beneficial effect on behavioral and histological outcomes.