Presynaptic MAST kinase controls opposing postsynaptic responses to convey stimulus valence in Caenorhabditis elegans
Presynaptic MAST kinase controls opposing postsynaptic responses to convey stimulus valence in Caenorhabditis elegans
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DOI：
10.1073/pnas.1909240117
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发表时间：
2020-01-21
影响因子：
11.1
通讯作者：
Mori, Ikue
中科院分区：
文献类型：
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作者：
Nakano, Shunji;Ikeda, Muneki;Mori, Ikue
突触前可塑性可以调节突触传递的强度。然而，尚不清楚突触前神经元的调节是否能引起兴奋性和抑制性突触后反应。我们在这里报道了秀丽隐杆线虫的MAST激酶、Stomatin激酶和二酰基甘油激酶的同源物在热感觉神经元中起作用，在其突触后神经元中引发与热刺激价相关的兴奋或抑制反应。通过监测自由行为动物的价编码中间神经元的神经活动，我们发现中间神经元兴奋和抑制反应之间的改变是通过控制突触前神经元释放的两种相反信号的平衡来调节的。这些可供选择的传输进一步产生相反的行为输出，这是在热梯度上导航所必需的。我们的研究结果表明，价格编码神经元间活动是由突触前机制决定的，其中MAST激酶、Stomatin和二酰基甘油激酶影响突触前输出。
Presynaptic plasticity is known to modulate the strength of synaptic transmission. However, it remains unknown whether regulation in presynaptic neurons can evoke excitatory and inhibitory postsynaptic responses. We report here that the Caenorhabditis elegans homologs of MAST kinase, Stomatin, and Diacylglycerol kinase act in a thermosensory neuron to elicit in its postsynaptic neuron an excitatory or inhibitory response that correlates with the valence of thermal stimuli. By monitoring neural activity of the valence-coding interneuron in freely behaving animals, we show that the alteration between excitatory and inhibitory responses of the interneuron is mediated by controlling the balance of two opposing signals released from the presynaptic neuron. These alternative transmissions further generate opposing behavioral outputs necessary for the navigation on thermal gradients. Our findings suggest that valence-encoding interneuronal activity is determined by a presynaptic mechanism whereby MAST kinase, Stomatin, and Diacylglycerol kinase influence presynaptic outputs.