Differential subcellular distribution of PC1, PC2 and furin in bovine adrenal medulla and secretion of PC1 and PC2 from this tissue.
Differential subcellular distribution of PC1, PC2 and furin in bovine adrenal medulla and secretion of PC1 and PC2 from this tissue.
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PC1、PC2 和弗林蛋白酶在牛肾上腺髓质中的差异亚细胞分布以及该组织中 PC1 和 PC2 的分泌。
DOI：
10.1016/0304-3940(92)90252-3
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发表时间：
1992
影响因子：
2.5
通讯作者：
Winkler,H
中科院分区：
文献类型：
--
作者：
Kirchmair,R;Egger,C;Gee,P;Hogue-Angeletti,R;Fischer-Colbrie,R;Laslop,A;Winkler,H
通过对密度梯度离心获得的级分进行免疫印迹，测定牛肾上腺髓质中 PC1、PC2 和弗林蛋白酶的亚细胞分布。发现 PC1 和 PC2 仅限于嗜铬颗粒，而这些细胞器中不存在弗林蛋白酶（C 端肽）。氯化氨基甲酰胆碱刺激牛肾上腺髓质诱导PC1和PC2的分泌。分泌的酶与嗜铬颗粒中的 PC1 和 PC2 具有相同的分子大小。
The subcellular distribution of PC1, PC2 and furin was determined in bovine adrenal medulla by immunoblotting of fractions obtained by density gradient centrifugation. PC1 and PC2 were found to be confined to chromaffin granules whereas furin (C-terminal-peptide) was absent from these organelles. Stimulation of bovine adrenal medulla by carbamoylcholine chloride induced the secretion of PC1 and PC2. The secreted enzymes had the same molecular size as PC1 and PC2 present in chromaffin granules.