Tobacco plants ectopically expressing the Ammopiptanthus mongolicus AmCBL1 gene display enhanced tolerance to multiple abiotic stresses
Tobacco plants ectopically expressing the Ammopiptanthus mongolicus AmCBL1 gene display enhanced tolerance to multiple abiotic stresses
复制标题
异位表达沙冬青 AmCBL1 基因的烟草植物表现出对多种非生物胁迫的耐受性增强
DOI：
10.1007/s10725-010-9523-4
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发表时间：
2011-04-01
影响因子：
4.2
通讯作者：
Yin, Wei-Lun
中科院分区：
文献类型：
--
作者：
Chen, Jin-Huan;Sun, Yan;Yin, Wei-Lun
Ca2+是导致高等植物胁迫基因表达的信号转导途径的关键成分，并调节广泛的生理过程。钙调神经磷酸酶b样蛋白（CBL）家族是植物中一类独特的钙传感器，在解码钙瞬态中起着关键作用。这里，一个CBL1同源基因AmCBL1 (NCBI加入号：采用逆转录聚合酶链反应（RT-PCR）和cDNA末端快速扩增（RACE）技术，从蒙古Ammopiptanthus mongolicus （Maxim.）中分离得到AY902246基因。实时荧光定量PCR （QRT-PCR）结果表明，干旱、高盐、高温和CaCl2处理显著诱导沙冬青幼苗AmCBL1表达。亚细胞定位分析表明AmCBL1是一个质膜定位蛋白。结果表明，AmCBL1不仅是沙冬青对盐胁迫的正调节因子，而且是温度胁迫的正调节因子。
Ca2+ is a critical component in signal transduction pathways that lead to stress gene expression in higher plants and regulates a wide range of physiological processes. The calcineurin B-like protein (CBL) family represents a unique group of calcium sensors in plants, and plays a key role in decoding calcium transients. Here, a CBL1 homolog gene, AmCBL1 (NCBI accession no. AY902246), was isolated from Ammopiptanthus mongolicus (Maxim.) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Quantitative real-time PCR (QRT-PCR) experiments showed that AmCBL1 was significantly induced by drought, high salinity, heat, and CaCl2 treatments in A. mongolicus seedlings. Subcellular localization analysis suggested that AmCBL1 is a plasma membrane-localized protein. Our results suggest that AmCBL1 is not only a positive regulator of salt response but also a positive regulator of temperature stresses in A. mongolicus.