Dentin remineralization via adhesive containing amorphous calcium phosphate nanoparticles in a biofilm-challenged environment
Dentin remineralization via adhesive containing amorphous calcium phosphate nanoparticles in a biofilm-challenged environment
复制标题
在生物膜挑战的环境中通过含有无定形磷酸钙纳米粒子的粘合剂进行牙本质再矿化
DOI：
10.1016/j.jdent.2019.103193
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发表时间：
2019
影响因子：
4.4
通讯作者：
Li Jiyao
中科院分区：
文献类型：
--
作者：
Tao Siying;He Libang;Xu Hockin H K;Weir Michael D;Fan Menglin;Yu Zhaohan;Zhang Min;Zhou Xuedong;Liang Kunneng;Li Jiyao
目的牙本质在粘结界面上的再矿化有助于强化粘结界面，抑制继发性龋病，延长修复体寿命。本研究的目的是研究无定形磷酸钙纳米颗粒粘结剂在牙科生物膜环境中对脱矿人牙本质的再矿化作用。方法将样品置于变形链球菌生物膜中24 h，促进牙本质脱矿。所有样本都接受再矿化程序，包括在脑心输液中每24小时暴露4小时，然后在剩余时间内浸泡在人工唾液中。每隔一天测一次浸泡4h后BHIS的pH值。10天后，对生物膜进行菌落形成单位(CFU)计数、乳酸产量、活/死染色以及钙和磷含量的评估。结果NACP粘结剂实现了酸中和，减少了生物膜菌落总数，减少了生物膜乳酸生成，增加了生物膜钙磷含量(P <； 0.0 5)。NACP粘结剂组的再矿化值高于商品氟释放粘结剂组(P <； 0.0 5)。结论在生物膜模型中，NACP粘结剂能有效地对牙本质病变进行再矿化。含NACP粘结剂在保护粘结界面、抑制继发性龋病、延长修复体寿命等方面具有广阔的应用前景。临床意义含NACP粘结剂的复合层即使在生物膜环境中也具有保护作用，值得推荐使用。
ObjectivesThe remineralization of dentin at a bonded interface would help to strengthen the bonded interface and inhibit secondary caries, and would prolong the longevity of restoration. The aim of this study was to investigate the remineralization of demineralized human dentin in a dental biofilm environment via an adhesive containing nanoparticles of amorphous calcium phosphate (NACP).MethodsDentin demineralization was promoted by subjecting samples to aStreptococcus mutansacidic biofilm for 24 h. Samples were divided into a control group, a commercial fluoride-releasing adhesive group, and an NACP adhesive group. All samples were subjected to a remineralization protocol consisting of 4-h exposure per 24-h period in brain heart infusion broth plus 1% sucrose (BHIS) followed by immersion in artificial saliva for the remaining period. The pH of BHIS after 4-h immersion was measured every other day. After 10 days, the biofilm was assessed for colony-forming unit (CFU) count, lactic acid production, live/dead staining, and calcium and phosphate content. The mineral changes in the demineralized dentin samples were analyzed by transverse microradiography, hardness measurement, X-ray diffraction characterization, and scanning electron microscopy.ResultsThe NACP adhesive achieved acid neutralization, decreased biofilm CFU count, decreased biofilm lactic acid production, and increased biofilm calcium and phosphate content (P < 0.05). The NACP adhesive group had higher remineralization value than the commercial fluoride-releasing adhesive group (P < 0.05).ConclusionsThe NACP adhesive was effective in remineralizing dentin lesions in a biofilm model. Its ability to protect bond interface, inhibit secondary caries, and prolong the longevity of restoration is promising.Clinical significanceUsing NACP-containing adhesives could be recommended because of the protective ability of its hybrid layer even under a biofilm-challenged environment.