The therapeutic effects of Periploca forrestii Schltr. Stem extracts on collagen-induced arthritis by inhibiting the activation of Src/NF-κB signaling pathway in rats
The therapeutic effects of Periploca forrestii Schltr. Stem extracts on collagen-induced arthritis by inhibiting the activation of Src/NF-κB signaling pathway in rats
复制标题
杠柳的治疗作用。
DOI：
10.1016/j.jep.2017.03.005
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发表时间：
2017-04-18
影响因子：
5.4
通讯作者：
Liu, Wenyuan
中科院分区：
文献类型：
--
作者：
Chen, Lei;Li, Jinsong;Liu, Wenyuan
民族药理相关性：香附。是一种经典的中医中药，名叫黑龙骨，在中国。根据中医理论，它具有祛风除湿的功效。临床上常用于治疗类风湿性关节炎。本研究旨在通过建立大鼠胶原诱导的类风湿关节炎(CIA)动物模型，评价HLG乙醇提取物的抗风湿作用，并揭示其可能的分子机制。材料和方法：在SD大鼠尾部皮内注射牛II型胶原蛋白和完全弗氏佐剂(CFA)，以诱导CIA。大鼠从第1天开始口服HLG(200 mg/kg和400 mg/kg)，共28天。测量了多种指标以评估抗风湿效果，包括足爪肿胀、关节炎评分和组织病理学变化。检测大鼠血清肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)、前列腺素E2(PGE2)水平及关节滑膜组织中环氧合酶-2(COX-2)、核因子-kappaBp65和Src激酶的水平，以探讨其可能的作用机制。同时，该治疗还显著降低了NF-kappa B p65和COX-2的表达。结论：HLG对大鼠胶原性关节炎有明显的治疗作用，其机制可能与抑制Src的激活和抑制核因子-kappaB的核转位有关。HLG可能是治疗类风湿关节炎的潜在候选药物。
Ethnopharmacological relevance: Periploca forrestii Schltr. is a classical traditional Chinese medicine (TCM) called Heilonggu (HLG) in China. According to the theory of TCM, it possesses the efficacy of eliminating wind and removing dampness. In clinical practice, it is commonly used for the treatment of rheumatoid arthritis. The present work aimed to evaluate the anti-rheumatism activity of HLG ethanol extract and reveal the underlying molecular mechanism by employing an animal model of collagen-induced rheumatoid arthritis (CIA) in rats.Materials and methods: The CIA was induced in male Sprague Dawley rats by intradermal injection of bovine collagen-II in complete Freund's adjuvant (CFA) at the base of tail. The rats received oral administration of HLG (200 and 400 mg/kg) from day 1, with the treatment lasting for 28 days. A variety of indicators were measured for evaluation of anti-rheumatism effect, including paw swelling, arthritis scores, and histopathological changes. Furthermore, the serum levels of tumor necrosis factor-alpha (TNF-alpha), interleuldn-6 (IL-6), and prostaglandin E2 (PGE2), as well as cyclooxygenase-2 (COX-2), nuclear factor NF-kappa B p65 and Src kinase in joint synovial tissues were detected to explore the possible mechanisms.Results: The administration of HLG significantly restored type II collagen-induced arthritis in rats as evidenced by decrease in paw swelling and inflammatory factors in serum. Meanwhile, this treatment also notably reduced NF-kappa B p65 and COX-2 expression. Surprisingly, the activity of Src kinase was also inhibited demonstrated by downregulation of phosphorylated Src.Conclusion: Our results revealed that HLG possessed observable therapeutic action on collagen-induced arthritis by inhibiting the activation of Src and nuclear translocation of NF-kappa B in rats. HLG may serve as a potential candidate for the management of patients with RA.