Adaptive Changes of the Yeast Mitochondrial Proteome in Response to Salt Stress
Adaptive Changes of the Yeast Mitochondrial Proteome in Response to Salt Stress
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DOI：
10.1089/omi.2010.0020
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发表时间：
2010-10-01
影响因子：
3.3
通讯作者：
Pascual-Ahuir, Amparo
中科院分区：
文献类型：
--
作者：
Martinez-Pastor, Mar;Proft, Markus;Pascual-Ahuir, Amparo
线粒体是动态细胞器，具有适应环境刺激和压力的能力。在这里，我们使用酵母（酿酒酵母）结合蛋白质组学方法来量化在 NaCl 引起的盐胁迫下线粒体蛋白质组成的变化。我们鉴定了 15 种蛋白质，它们在盐适应线粒体中的表达量超过两倍。这些蛋白质主要参与氧化应激防御、氨基酸和泛醌的生物合成或丙酮酸和乙酸的代谢。大多数上调蛋白的功能丧失在高盐条件下不会导致显着的生长表型。然而，所有已鉴定的蛋白质都是在过氧化氢引起的氧化应激下维持有效生长所必需的。此外，线粒体外膜蛋白的一个子集在盐胁迫下被上调。我们还鉴定了九种蛋白质，它们在盐适应线粒体中的代表性不足三倍以上。这些蛋白质主要是糖酵解酶或主要定位于内质网的蛋白质。我们的结果强调了线粒体应激适应的复杂性，并确定了蛋白质的功能组，其在耐盐性中的具体作用应该在未来被揭示。
Mitochondria are dynamic organelles with the capacity to adapt to environmental stimuli and stress. Here we use yeast (Saccharomyces cerevisiae) in combination with proteomic approaches to quantify the changes in the protein composition of mitochondria in the presence of salt stress provoked by NaCl. We identified 15 proteins that were more than twofold overrepresented in salt adapted mitochondria. These proteins are mainly involved in the oxidative stress defense, the biosynthesis of amino acids and ubiquinone or in the metabolism of pyruvate and acetate. Loss of function of most of the upregulated proteins did not result in a significant growth phenotype under high salt conditions. However, all identified proteins were necessary to sustain efficient growth under oxidative stress caused by hydrogen peroxide. Additionally, a subset of outer mitochondrial membrane proteins was shown to be upregulated upon salt stress. We furthermore identified nine proteins that were more than threefold underrepresented in salt adapted mitochondria. These proteins were mainly glycolytic enzymes or proteins with a predominant localization at the endoplasmatic reticulum. Our results underline the complex nature of the stress adaptation of mitochondria and identify functional groups of proteins whose specific role in salt resistance should be revealed in the future.