Angiotensin-converting enzyme 2 (ACE2) proteins of different bat species confer variable susceptibility to SARS-CoV entry.
Angiotensin-converting enzyme 2 (ACE2) proteins of different bat species confer variable susceptibility to SARS-CoV entry.
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DOI：
10.1007/s00705-010-0729-6
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发表时间：
2010-10
影响因子：
2.7
通讯作者：
Shi Z
中科院分区：
文献类型：
--
作者：
Hou Y;Peng C;Yu M;Li Y;Han Z;Li F;Wang LF;Shi Z
在蝙蝠身上发现的SARS样冠状病毒表明，蝙蝠可能是SARS冠状病毒的天然宿主。然而，以前的研究表明，血管紧张素转换酶2（ACE 2）蛋白，一个已知的SARS冠状病毒受体，从马蹄蝙蝠不能作为SARS冠状病毒的功能性受体。在这里，我们将我们以前的研究扩展到来自另外七种蝙蝠的ACE 2分子，并使用基于HIV的假型和活SARS-CoV感染检测来测试它们与人类SARS-CoV刺突蛋白的相互作用。结果表明，鼠耳蝠和中华菊头蝠的ACE 2支持由SARS-CoV S蛋白介导的病毒进入，尽管与人ACE 2相比具有不同的效率。此外，几个关键残基的改变降低或增强蝙蝠ACE 2受体的效率，从不同蝙蝠ACE 2分子的结构建模研究预测。这些数据表明M. daughoni和R.中华绒螯蟹对SARS-CoV易感，可能是SARS-CoV的天然宿主。此外，我们目前的研究还表明，蝙蝠中ACE 2的遗传多样性大于已知的SARS冠状病毒易感哺乳动物中观察到的遗传多样性，这突出了可能有更多的未知蝙蝠物种可以作为SARS冠状病毒或其祖先病毒的储存库。这就要求继续和扩大对不同蝙蝠种群的实地监测研究，以最终确定SARS冠状病毒的真正天然宿主。本文的在线版本（doi：10.1007/s 00705 -010-0729-6）包含补充材料，可供授权用户使用。
The discovery of SARS-like coronavirus in bats suggests that bats could be the natural reservoir of SARS-CoV. However, previous studies indicated the angiotensin-converting enzyme 2 (ACE2) protein, a known SARS-CoV receptor, from a horseshoe bat was unable to act as a functional receptor for SARS-CoV. Here, we extended our previous study to ACE2 molecules from seven additional bat species and tested their interactions with human SARS-CoV spike protein using both HIV-based pseudotype and live SARS-CoV infection assays. The results show that ACE2s of Myotis daubentoni and Rhinolophus sinicus support viral entry mediated by the SARS-CoV S protein, albeit with different efficiency in comparison to that of the human ACE2. Further, the alteration of several key residues either decreased or enhanced bat ACE2 receptor efficiency, as predicted from a structural modeling study of the different bat ACE2 molecules. These data suggest that M. daubentoni and R. sinicus are likely to be susceptible to SARS-CoV and may be candidates as the natural host of the SARS-CoV progenitor viruses. Furthermore, our current study also demonstrates that the genetic diversity of ACE2 among bats is greater than that observed among known SARS-CoV susceptible mammals, highlighting the possibility that there are many more uncharacterized bat species that can act as a reservoir of SARS-CoV or its progenitor viruses. This calls for continuation and expansion of field surveillance studies among different bat populations to eventually identify the true natural reservoir of SARS-CoV. The online version of this article (doi:10.1007/s00705-010-0729-6) contains supplementary material, which is available to authorized users.
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DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
影响因子：
11.4
作者：
Li, WH;Zhang, CS;Sui, JH;Kuhn, JH;Moore, MJ;Luo, SW;Wong, SK;Huang, IC;Xu, KM;Vasilieva, N;Murakami, A;He, YQ;Marasco, WA;Guan, Y;Choe, HY;Farzan, M
通讯作者：
Farzan, M
影响因子：
11.8
作者：
Tu C;Crameri G;Kong X;Chen J;Sun Y;Yu M;Xiang H;Xia X;Liu S;Ren T;Yu Y;Eaton BT;Xuan H;Wang LF
通讯作者：
Wang LF
影响因子：
11.8
作者：
Cui J;Han N;Streicker D;Li G;Tang X;Shi Z;Hu Z;Zhao G;Fontanet A;Guan Y;Wang L;Jones G;Field HE;Daszak P;Zhang S
通讯作者：
Zhang S
SARS冠状病毒尖峰和核皮质蛋白的免疫主导区域的确定和应用由不同动物物种的血清识别。
影响因子：
2.2
作者：
Yu M;Stevens V;Berry JD;Crameri G;McEachern J;Tu C;Shi Z;Liang G;Weingartl H;Cardosa J;Eaton BT;Wang LF
通讯作者：
Wang LF