Activation of mouse lymphocytes by anti-immunoglobulin. I. Parameters of the proliferative response.
Activation of mouse lymphocytes by anti-immunoglobulin. I. Parameters of the proliferative response.
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DOI：
10.1084/jem.147.3.814
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发表时间：
1978-03-01
期刊：
影响因子：
--
通讯作者：
Paul WE
中科院分区：
文献类型：
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作者：
Sieckmann DG;Asofsky R;Mosier DE;Zitron IM;Paul WE
通过山羊抗小鼠IgM抗血清和从该血清制备的纯化抗mu抗体刺激来自各种品系的年轻成年小鼠的脾细胞培养物以掺入氚化胸苷（[3 H]TdR）。这种刺激被证明取决于抗血清的抗mu活性。此外，超浓缩的抗-mu和抗-mu的F（ab '）2片段显示出刺激性。抗mu制剂缺乏可检测的内毒素污染，并且还显示出刺激对内毒素的促有丝分裂作用无反应的两种菌株（C57 BL/10 ScCr和C3 H/HeJ）的反应，而它不能刺激来自对内毒素有反应的小鼠菌株（CBA/N）的细胞的反应。此外，纯化的山羊抗小鼠γ、κ抗体和兔抗小鼠κ抗体刺激小鼠脾细胞摄取[3 H]TdR，但程度低于抗μ制剂。细胞密度，培养要求，和动力学的反应。
Spleen cell cultures from young adult mice of a variety of strains were stimulated to incorporate tritiated thymidine ([3H]TdR) by a goat anti- mouse IgM antiserum and by purified anti-mu antibodies prepared from this serum. This stimulation was shown to depend upon the anti-mu activity of the antiserum. In addition, ultracentrifuged anti-mu and F(ab')2 fragments of anti-mu were shown to be stimulatory. The anti-mu preparation lacked detectable endotoxin contamination and was also shown to stimulate response by two strains (C57BL/10ScCr and C3H/HeJ) which are unresponsive to the mitogenic effects of endotoxin, while it failed to stimulate a response by cells from a mouse strain (CBA/N) which responds to endotoxin. In addition purified goat anti-mouse gamma, kappa antibodies and rabbit anti-mouse kappa-antib odies stimulated uptake of [3H]TdR by mouse spleen cells, although to a lesser degree than the anti-mu preparation. The cell density, culture requirements, and kinetics of the response are presented.