PROTEIN ADSORPTION AND MACROPHAGE ACTIVATION ON POLYDIMETHYLSILOXANE AND SILICONE-RUBBER
PROTEIN ADSORPTION AND MACROPHAGE ACTIVATION ON POLYDIMETHYLSILOXANE AND SILICONE-RUBBER
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DOI：
10.1163/156856295x00670
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发表时间：
1995-01-01
影响因子：
3.6
通讯作者：
BONFIELD, TL
中科院分区：
文献类型：
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作者：
ANDERSON, JM;ZIATS, NP;BONFIELD, TL
对聚二甲基硅氧烷、PDMS和硅橡胶的静态和动态人体血液吸附研究表明，这些材料在蛋白质吸附行为方面相似，但不完全相同。纤维蛋白原、免疫球蛋白G和白蛋白是材料表面上鉴定的主要蛋白质，纤连蛋白、Hageman因子（因子XII）和因子VIII/vWF以中等水平吸附。虽然两种材料的蛋白质吸附特性相似，但与PDMS相比，在硅橡胶上鉴定出的相应蛋白质水平更高。在PDMS、硅橡胶和低密度聚乙烯（LDPE）上孵育的单核细胞/巨噬细胞（有或没有蛋白质吸附）产生了不同水平的IL-1 β、IL-6和TNF-α，这取决于聚合物和吸附的蛋白质。与聚苯乙烯对照和聚乙烯相比，PDMS显示出较低水平的细胞因子。PDMS，聚苯乙烯和LDPE表面上的蛋白质预吸附显示出较低水平的细胞因子相比，没有蛋白质吸附产生的各自的数量表明钝化作用的蛋白质吸附现象对单核细胞/巨噬细胞活化。IgG、纤维蛋白原或纤连蛋白的预吸附降低了IL-1 β的定量表达，但增加了胸腺细胞增殖试验中的功能活性，表明存在单核细胞/巨噬细胞活化产物，其以独立的方式下调IL-1 β的活性或上调胸腺细胞增殖。
Static and dynamic human blood adsorption studies on polydimethylsiloxane, PDMS, and silicone rubber show that these materials are similar, but not identical, in their protein adsorption behavior. Fibrinogen, immunoglobulin G, and albumin were the predominant proteins identified on the material surfaces with fibronectin, Hageman factor (factor XII), and factor VIII/vWF adsorbing at intermediate levels. While the protein adsorption characteristics for the two materials were similar, higher levels of the respective proteins were identified on silicone rubber compared to PDMS. Monocytes/macrophages incubated on PDMS, silicone rubber and low density polyethylene, LDPE, with or without protein adsorption produced variable levels of IL-I beta, IL-6 and TNF-alpha dependent on the polymer and adsorbed protein. PDMS showed lower levels of the cytokines when compared to the polystyrene control and polyethylene. Protein preadsorption on the PDMS, polystyrene, and LDPE surfaces showed lower levels of cytokines when compared to the respective quantities produced with no protein adsorption suggesting a passivating effect by the protein adsorption phenomenon on monocyte/macrophage activation. Preadsorption of IgG, fibrinogen or fibronectin decreased the quantitative expression of IL-1 beta but increased the functional activity in the thymocyte proliferation assay indicating the presence of monocyte/macrophage activation products which either downregulated the activity of IL-1 beta or upregulated thymocyte proliferation in an independent fashion.