Direct antioxidant and protective effect of estradiol on isolated mitochondria
Direct antioxidant and protective effect of estradiol on isolated mitochondria
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DOI：
10.1016/j.bbadis.2009.09.007
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发表时间：
2010-01-01
影响因子：
6.2
通讯作者：
Vina, Jose
中科院分区：
文献类型：
--
作者：
Borras, Consuelo;Gambini, Juan;Vina, Jose
雌激素具有抗氧化特性，这是由于它们能够与雌激素受体结合，并通过细胞内信号通路上调抗氧化酶的表达。线粒体是衰老相关细胞损伤发生的关键细胞器。最近，在线粒体中发现了雌激素受体。本文的目的是测试雌二醇是否通过防止氧化应激和保护脆弱的线粒体直接影响线粒体。用正常细胞内浓度的雌二醇孵育可防止线粒体以饱和方式形成活性氧。此外，雌二醇保护线粒体完整性，这表明线粒体膜电位的增加。它还可以防止线粒体细胞色素c的凋亡性渗漏，从而使线粒体细胞色素c的含量保持在较高水平。因此，雌二醇通过对细胞器的直接作用来阻止线粒体凋亡途径的发生。染料木素是一种高浓度存在于大豆中的植物雌激素，它通过降低活性氧的形成速度和阻止线粒体释放细胞色素c来模仿雌二醇的保护作用。(C) 2009 Elsevier B.V.版权所有
Estrogens have antioxidant properties which are due to their ability to bind to estrogen receptors and to upregulate the expression of antioxidant enzymes via intracellular signalling pathways. Mitochondria are key organelles in the development of age-associated cellular damage. Recently, estrogen receptors were identified in mitochondria. The aim of this paper was to test whether estradiol directly affects mitochondria by preventing oxidative stress and protecting frail mitochondria. Incubation with estradiol at normal intracellular concentrations prevents the formation of reactive oxygen species by mitochondria in a saturable manner. Moreover, estradiol protects mitochondrial integrity as indicated by an increase in mitochondrial membrane potential. It also prevents the apoptogenic leakage of cytochrome c from mitochondria and as a result the mitochondrial content of this cytochrome c is maintained high. Thus, estradiol prevents the onset of the mitochondrial pathway of apoptosis by a direct effect on the organelle. Genistein, a phytoestrogen present at high concentration in soy, mimics the protective effect of estradiol by both decreasing the rate of formation of reactive oxygen species and preventing the release of cytochrome c from mitochondria. (C) 2009 Elsevier B.V. All rights reserved.