SINGLE INTRACEREBRAL INJECTION OF APOTRANSFERRIN IN YOUNG-RATS INDUCES INCREASED MYELINATION
SINGLE INTRACEREBRAL INJECTION OF APOTRANSFERRIN IN YOUNG-RATS INDUCES INCREASED MYELINATION
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DOI：
10.1159/000112116
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发表时间：
1994-09-01
影响因子：
2.9
通讯作者：
PASQUINI, JM
中科院分区：
文献类型：
--
作者：
CABRERA, OEE;BONGARZONE, ER;PASQUINI, JM
三日龄大鼠颅内注射210或350 ng的脱铁转铁蛋白（aTf），以研究这种铁转运蛋白可能的神经营养作用。给处理动物和适当的对照动物注射生理盐水、变性aTf或卵清蛋白。从脑中分离髓鞘，并用于研究其化学组成和蛋白质电泳图谱。总髓鞘半乳糖脂，特别是总磷脂显着增加，在这两个年龄段的研究与对照组。还观察到总胆固醇轻微增加。与对照组相比，总髓鞘蛋白在第10天和第17天均显著增加。氯仿：甲醇可溶性蛋白质（蛋白脂质）仅略有增加。电泳图谱显示，在处理的动物中，对应于髓鞘碱性蛋白（MBP）的两个主要条带相对增加。2 '3'-环核苷酸3 '-磷酸水解酶（CNNH 3）的酶活性显著提高。观察到的变化具有剂量和年龄依赖性。注射aTf仅在短的发育期内有效，因为在20日龄时治疗的动物显示髓鞘组成没有明显变化。aTf注射的作用持续至少长达60天，因为在这个年龄，从注射的动物中获得的髓鞘与对照动物相比仍然含有更高量的总蛋白质磷脂和半乳糖脂。aTf的作用似乎对髓磷脂具有特异性，因为在全脑匀浆的膜中没有观察到显著的作用。由于其他蛋白质（卵清蛋白）或热变性的aTf无效，因此注射的脱辅基蛋白具有特异性作用。
Three-day-old rats were injected intracranially with 210 or 350 ng of apotransferrin (aTf) to study the possible neurotrophic effects of this iron transport protein. Treated animals and appropriate controls were injected with saline, denatured aTf or ovalbumin. Myelin was isolated from the brains and used to study its chemical composition and the protein electrophoretic pattern. Total myelin galactolipids and especially total phospholipids were significantly increased with reference to controls at both ages studied. A slight increase in total cholesterol was also observed. Total myelin proteins were markedly increased both at 10 and 17 days in comparison to controls. Chloroform:methanol-soluble proteins (proteolipids) were only slightly increased. The electrophoretic profile showed that the two main bands corresponding to myelin basic protein (MBP) were relatively increased in the treated animals. The enzymatic activity of 2'3'-cyclic nucleotide 3'-phosphohydrolase (CNPase) was significantly increased. The changes observed were dose- and age-dependent. The injection of aTf was effective only within a short developmental period since animals treated at 20 days of age showed no apparent changes in myelin composition. The actions of aTf injection persisted at least up to 60 days, since at this age the myelin obtained from injected animals still contained higher amounts of total proteins phospholipids and galactolipids in comparison to control animals. The action of aTf appears to be specific for myelin, since no significant effects were observed in membranes of a total brain homogenate. The effects are specific for the apoprotein injected since other proteins (ovalbumin) or heat-denatured aTf were ineffective.