MEASUREMENT OF CELL GROWTH IN TISSUE CULTURE WITH A PHENOL REAGENT (FOLIN-CIOCALTEAU)
MEASUREMENT OF CELL GROWTH IN TISSUE CULTURE WITH A PHENOL REAGENT (FOLIN-CIOCALTEAU)
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DOI：
10.3181/00379727-91-22245
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发表时间：
1956-01-01
影响因子：
--
通讯作者：
EAGLE, H
中科院分区：
文献类型：
--
作者：
OYAMA, VI;EAGLE, H
描述了一种测量组织培养中细胞生长的分析方法，该方法基于用于测定蛋白质的 Lowry 方法，并使用酚试剂 (Folin-Ciocalteau) 进行显色。参考牛血清白蛋白标准品的结果可以通过适当的转换因子转换为干重、氮或细胞计数。这些因素针对 7 种人类细胞系和 1 种小鼠细胞系给出。
An analytical method for the measurement of cell growth in tissue culture is described, based on the Lowry method for the determination of protein, and employing a phenol reagent (Folin-Ciocalteau) for color development. The results, referred to a bovine serum albumin standard, may be converted to dry weight, nitrogen or cell count by appropriate conversion factors. Those factors are given for 7 human cell lines and 1 mouse line.