Cardiac-specific inducible and conditional gene targeting in mice.
Cardiac-specific inducible and conditional gene targeting in mice.
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DOI：
10.1161/circresaha.112.265066
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发表时间：
2012-05-25
影响因子：
20.1
通讯作者：
Azhar M
中科院分区：
文献类型：
--
作者：
Doetschman T;Azhar M
小鼠基因工程彻底改变了我们对心脏发育和疾病的分子和遗传基础的理解。该技术涉及有条件的组织特异性和时间性转基因和基因靶向方法，以及将多态性引入小鼠基因组。这些方法越来越多地用于阐明成人心脏组织稳态、生理学和病理生理学的遗传途径。它们还导致了人类心脏病临床相关模型的发展。在这里，我们回顾了技术及其局限性，特别是心血管研究界。
Mouse genetic engineering has revolutionized our understanding of the molecular and genetic basis of heart development and disease. This technology involves conditional tissue-specific and temporal transgenic and gene targeting approaches, as well as introduction of polymorphisms into the mouse genome. These approaches are increasingly used to elucidate the genetic pathways underlying tissue homeostasis, physiology, and pathophysiology of adult heart. They have also led to the development of clinically relevant models of human cardiac diseases. Here, we review the technologies and their limitations in general and the cardiovascular research community in particular.