Determination of cyclopeptide toxins in Amanita subpallidorosea and Amanita virosa by high-performance liquid chromatography coupled with high-resolution mass spectrometry
Determination of cyclopeptide toxins in Amanita subpallidorosea and Amanita virosa by high-performance liquid chromatography coupled with high-resolution mass spectrometry
复制标题
高效液相色谱-高分辨率质谱法测定亚苍白鹅膏菌和病毒鹅膏菌中的环肽毒素
DOI：
10.1016/j.toxicon.2017.04.012
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发表时间：
2017-07-01
期刊：
影响因子：
2.8
通讯作者：
Xie, Jianwei
中科院分区：
文献类型：
--
作者：
Wei, Jiahui;Wu, Jianfeng;Xie, Jianwei
亚苍白鹅膏菌是新近发现的一种致死性鹅膏菌。根据分子系统发育分析，在中国发现的与病毒菌属聚在同一林地的蝴蝶亚科植物。然而，对这些致死蘑菇的环肽毒素含量的研究还很少。本研究首次报道了亚苍白球藻中的环肽毒素，并系统分析了亚苍白球藻和弧菌的环肽组成。采用高效液相色谱-高分辨质谱联用技术，从这两种致死蘑菇中鉴定出13个环肽类化合物和2个未知化合物。在已知的环肽中，以前被认为仅限于病原菌的病毒毒素丙氨酸、病毒素和病毒素在亚苍白僵菌中被鉴定出来。环肽组成分析表明，不同种类和不同病原菌的鹅膏毒素、鬼臼毒素和病毒毒素的种类和含量存在差异，且供试种的总毒素含量显著高于供试病原菌。此外，环肽毒素与分子系统发育关系的一致性被证明。(C)2017爱思唯尔有限公司。保留所有权利。
Amanita subpallidorosea is a recently discovered lethal Amanita sect. Phalloideae species found in China that is clustered with A. virosa in the same Glade based on molecular phylogenetic analysis. However, the cyclo-peptide toxin contents of these lethal mushrooms remain poorly studied. In this study, the cyclopeptide toxins in A. subpallidorosea were reported for the first time and the cyclopeptide compositions of A. subpallidorosea and A. virosa species were systematically analyzed. Thirteen cyclopeptides and two unknown compounds were identified or observed from these two lethal mushrooms by high-performance liquid chromatography coupled with high-resolution mass spectrometry. Of the known cyclopeptides, the virotoxins alaviroidin, viroisin, and viroidin, which were previously thought to be restricted to A. virosa, were identified in A. subpallidorosea. The cyclopeptide compositions showed that there are diversities in the kinds and levels of amatoxins, phallotoxins, and virotoxins between A. subpallidorosea and A. virosa species, and that the amount of total toxins in the tested A. subpallidorosea is significantly higher than that in the tested A. virosa. Furthermore, consistency of the cyclopeptide toxins with the molecular phylogenetic relationships was demonstrated. (C) 2017 Elsevier Ltd. All rights reserved.