Field, Genetic, and Modeling Approaches Show Strong Positive Selection Acting upon an Insecticide Resistance Mutation in Anopheles gambiae s.s.
Field, Genetic, and Modeling Approaches Show Strong Positive Selection Acting upon an Insecticide Resistance Mutation in Anopheles gambiae s.s.
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DOI：
10.1093/molbev/msq002
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发表时间：
2010-05-01
影响因子：
10.7
通讯作者：
Donnelly, Martin J.
中科院分区：
文献类型：
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作者：
Lynd, Amy;Weetman, David;Donnelly, Martin J.
最近受到强有力的正选择的等位基因将与基因组的连续片段一起被扫向固定。目前还不清楚这种选择的基因组特征是否会在远缘繁殖的野生种群中容易检测到。在这项研究中，我们使用单倍型多样性分析来检验选择性扫除与冈比亚按蚊对二氯二苯基三氯乙烷和拟除虫菊酯类杀虫剂抗药性相关的击倒抗性(Kdr)突变的证据。这两个kdr突变的单倍型多样性都显著低于野生型(非抗性)等位基因，其中kdr L1014F表现出最明显的选择足迹。我们用收集的时间序列来补充这些数据，显示L1014F等位基因的频率在5年内从0.05增加到0.54，与最大似然拟合选择系数0.16和显性系数0.25一致。我们的数据表明，在野生昆虫种群中可以识别出强烈的、最近的积极选择事件，例如那些由杀虫剂抗药性引起的事件。
Alleles subject to strong, recent positive selection will be swept toward fixation together with contiguous sections of the genome. Whether the genomic signatures of such selection will be readily detectable in outbred wild populations is unclear. In this study, we employ haplotype diversity analysis to examine evidence for selective sweeps around knockdown resistance (kdr) mutations associated with resistance to dichlorodiphenyltrichloroethane and pyrethroid insecticides in the mosquito Anopheles gambiae. Both kdr mutations have significantly lower haplotype diversity than the wild-type (nonresistant) allele, with kdr L1014F showing the most pronounced footprint of selection. We complement these data with a time series of collections showing that the L1014F allele has increased in frequency from 0.05 to 0.54 in 5 years, consistent with a maximum likelihood-fitted selection coefficient of 0.16 and a dominance coefficient of 0.25. Our data show that strong, recent positive selective events, such as those caused by insecticide resistance, can be identified in wild insect populations.