Engagement of GPI-linked CD48 contributes to TCR signals and cytoskeletal reorganization: A role for lipid rafts in T cell activation
Engagement of GPI-linked CD48 contributes to TCR signals and cytoskeletal reorganization: A role for lipid rafts in T cell activation
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DOI：
10.1016/s1074-7613(00)80644-5
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发表时间：
1998-12-01
期刊：
影响因子：
32.4
通讯作者：
Miceli, MC
中科院分区：
文献类型：
--
作者：
Moran, M;Miceli, MC
GPI连接的蛋白质与细胞内酪氨酸激酶在“脂筏”中共同作用，被认为是信号转导和细胞骨架重组的平台。TCR活化需要酪氨酸激酶信号和细胞骨架重组。受体参与如何启动细胞骨架的变化仍然知之甚少。我们研究了招募GPI连接的CD 48和相关筏的T细胞的网站：APC接触刺激T细胞与表达CD 48配体CD 2的APC的后果。我们证明，CD 2：CD 48相互作用增强TCR介导的功能。CD 48/TCR共结合定性和定量地增强了脂筏依赖性5与肌动蛋白细胞骨架和5酪氨酸磷酸化的结合。这意味着脂筏作为受体诱导的信号和细胞骨架重组整合的位点，并揭示了辅助分子功能的新组分。
GPI-linked proteins coassociate with intracellular tyrosine kinases in "lipid rafts" proposed to function as platforms for signal transduction and cytoskeletal reorganization. TCR activation requires both tyrosine kinase signals and cytoskeletal reorganization. How receptor engagement initiates cytoskeletal changes remains poorly understood. We investigated the consequences of recruiting GPI-linked CD48 and associated rafts to the site of T cell:APC contact by stimulating T cells with APCs that express the CD48 ligand CD2. We demonstrate that CD2:CD48 interactions enhance TCR-mediated functions. CD48/TCR coengagement qualitatively and quantitatively enhances lipid raft-dependent 5 association with the actin cytoskeleton and 5 tyrosine phosphorylation. This implicates lipid rafts as sites where receptor-induced signals and cytoskeletal reorganization are integrated and reveals a novel component of accessory molecule function.