Intracellular delivery of core-shell fluorescent silica nanoparticles
Intracellular delivery of core-shell fluorescent silica nanoparticles
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DOI：
10.1016/j.biomaterials.2007.11.025
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发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Langer, Robert S.
中科院分区：
文献类型：
--
作者：
Fuller, Jason E.;Zugates, Gregory T.;Langer, Robert S.
通过改进的斯托伯法(C点)制备的高荧光核壳二氧化硅纳米颗粒有望作为亚细胞试剂和结构的传感和成像工具，但只有当它们能够容易地输送到受试者细胞的细胞质中时才会有用。这项工作表明，C点可以被阳离子聚合物静电覆盖，改变它们的表面电荷，使它们能够从内体逃逸，进入细胞质和细胞核。作为细胞递送的一个例子，我们证明了这些颗粒也可以与DNA络合，并介导和示踪DNA递送和基因表达。(C)2007年由爱思唯尔有限公司出版。
Highly fluorescent core-shell silica nanoparticles made by the modified Stober process (C dots) are promising as tools for sensing and imaging subcellular agents and structures but will only be useful if they can be easily delivered to the cytoplasm of the subject cells. This work shows that C dots can be electrostatically coated with cationic polymers, changing their surface charge and enabling them to escape from endosomes and enter the cytoplasm and nucleus. As an example of cellular delivery, we demonstrate that these particles can also be complexed with DNA and mediate and trace DNA delivery and gene expression. (c) 2007 Published by Elsevier Ltd.