Evolution of the Adh locus in the Drosophila willistoni group: the loss of an intron, and shift in codon usage.
Evolution of the Adh locus in the Drosophila willistoni group: the loss of an intron, and shift in codon usage.
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DOI：
10.1093/oxfordjournals.molbev.a040027
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发表时间：
1993-05
影响因子：
10.7
通讯作者：
Cort L. Anderson;Elizabeth A. Carew;Jeff R. Powell
中科院分区：
文献类型：
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作者：
Cort L. Anderson;Elizabeth A. Carew;Jeff R. Powell
我们在这里报告的DNA序列的醇脱氢酶基因（Adh）克隆从果蝇willistoni。主要研究结果如下：（1）相对于果蝇中已知的Adh基因，D. willistoni Adh的最后一个内含子被精确删除;直接从总基因组DNA进行的PCR表明，willistoni组的所有成员中都存在这种缺失，但在任何其他组中都不存在，包括密切相关的saltans组。除此之外，结构和预测的蛋白质与其他物种的非常相似。(2)与D.黑腹脂鲤最引人注目的转变是摆动位置（第三位置和第一位置）从C到U。与D. melanogaster，包括Adh，D. willistoni在第三位拥有近50%的G + C。(3)这个新序列提供的系统发育信息与几乎所有其他分子和形态学数据一致，将obscura组更接近melanogaster组，willistoni组更远，但仍然清楚地在Sophophora亚属内。
We report here the DNA sequence of the alcohol dehydrogenase gene (Adh) cloned from Drosophila willistoni. The three major findings are as follows: (1) Relative to all other Adh genes known from Drosophila, D. willistoni Adh has the last intron precisely deleted; PCR directly from total genomic DNA indicates that the deletion exists in all members of the willistoni group but not in any other group, including the closely related saltans group. Otherwise the structure and predicted protein are very similar to those of other species. (2) There is a significant shift in codon usage, especially compared with that in D. melanogaster Adh. The most striking shift is from C to U in the wobble position (both third and first position). Unlike the codon-usage-bias pattern typical of highly biased genes in D. melanogaster, including Adh, D. willistoni has nearly 50% G + C in the third position. (3) The phylogenetic information provided by this new sequence is in agreement with almost all other molecular and morphological data, in placing the obscura group closer to the melanogaster group, with the willistoni group farther distant but still clearly within the subgenus Sophophora.