Overexpression of AtbHLH112 suppresses lateral root emergence in Arabidopsis.
Overexpression of AtbHLH112 suppresses lateral root emergence in Arabidopsis.
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DOI：
10.1071/fp13253
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发表时间：
2014-04
期刊：
影响因子：
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通讯作者：
Wen-Shu Wang;Jiang Zhu;Ying‐Tang Lu
中科院分区：
文献类型：
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作者：
Wen-Shu Wang;Jiang Zhu;Ying‐Tang Lu
碱性/螺旋-环-螺旋（basic/helix-loop-helix，bHLH）转录因子是真核生物中普遍存在的一种转录调节因子，控制着许多不同的发育和生理过程。在这项研究中，AtbHLH 112，在拟南芥中的bHLH家族的未表征的成员的功能进行了研究。AtbHLH 112的过表达抑制了拟南芥幼苗侧根的发育。在显微镜下观察发现，内胚层中保留有4层以上细胞层的平头型异常侧根原基占侧根原基和侧根总数的45%以上。这表明，在转基因品系中，在LRP穿透皮层层之前，LRP的出现被阻止。LRP和LRP周围的亲本根细胞内生长素水平降低，以及根中细胞壁重塑（CWR）基因的表达下调可能有助于抑制AtbHLH 112过表达株系中LR的出现。这一发现进一步支持的观察，外源应用生长素恢复LR的发展和上调表达的CWR基因AtbHLH 112过表达线。
The basic/helix-loop-helix (bHLH) transcription factors are ubiquitous transcriptional regulators that control many different developmental and physiological processes in the eukaryotic kingdom. In this study, the function of AtbHLH112, an uncharacterised member of the bHLH family in Arabidopsis was investigated. Overexpression of AtbHLH112 suppressed lateral root (LR) development in Arabidopsis seedlings. Examination under the microscope revealed that abnormal lateral root primordia (LRP) with flat-head and more than four cell layers retained in the endodermal layer account for over 45% of the total number of LRP and LRs. This suggests that LRP emergence was prevented before LRP penetrated the cortical layer in the transgenic lines. Decreased auxin level within the LRP and parental root cells surrounding the LRP, as well as downregulated expression of cell-wall-remodelling (CWR) genes in the roots may contribute to the suppression of LR emergence in AtbHLH112-overexpressing lines. This finding was further supported by the observation that exogenous application of auxin recovered LR development and upregulated the expression of CWR genes in AtbHLH112-overexpressing lines.