c-Phycocyanin primed silver nano conjugates: Studies on red blood cell stress resilience mechanism
c-Phycocyanin primed silver nano conjugates: Studies on red blood cell stress resilience mechanism
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DOI：
10.1016/j.colsurfb.2020.111211
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发表时间：
2020-10-01
影响因子：
5.8
通讯作者：
Maruyama, Masugi
中科院分区：
文献类型：
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作者：
Madhyastha, Harishkumar;Madhyastha, Radha;Maruyama, Masugi
绿色合成的金属包裹的营养纳米杂化物已成为过去几年的研究目标。在本研究中，我们报道了使用FDA批准的藻蓝蛋白（cPC）的银纳米颗粒的温度依赖性的简易合成。采用TEM、Zeta电位、UV-vis、XPS、FTIR和CD光谱等对cPC结合银纳米粒子（AgcPCNPs）进行了表征。温度优化研究表明，在40 ℃下合成稳定的AgcPCNP，而在更高的温度下，系统显示聚集的外观。分子对接研究预测了cPC的C、D、I和J链与AgNP表面的排他性相互作用。此外，AgcPCNP通过测量参数如总RBC计数、%溶血、%血细胞比容、凝血时间、pH、电解质浓度和通过抗氧化机制的血细胞脂质过氧化程度来显著（p <0.1%）抵消AgNP对红细胞的毒性性质。皮肤成纤维细胞在体外细胞迁移的结果表明，AgcPCNPs增强了细胞向伤口区域的运动程度。所获得的数据共同表明，AgcPCNP可以是皮肤伤口愈合中的更好的试剂，具有降低的毒性，具有cPC作为伤口愈合剂和Ag纳米金属作为抗菌剂的双相优势。
Green synthesis of metal-encased nutraceutical nano-hybrids has been a target for research over the last few years. In the present investigation, we have reported temperature dependent facile synthesis of silver nanoparticles using FDA approved c phycocyanin (cPC). The cPC conjugated silver nanoparticles (AgcPCNPs) were characterized by TEM, Zeta Potential, UV-vis, XPS, FTIR, and CD Spectroscopy. The temperature optimization studies suggested the synthesis of stable AgcPCNPs at 40 degrees C while at higher temperature system shows aggregated appearance. Molecular docking studies predicted the exclusive interaction of C, D, I, and J chains of cPC with the surface of AgNPs. Moreover, AgcPCNPs significantly (p < 0.1 %) counteract the toxic nature of AgNPs on red blood cell by measuring parameters like total RBC count, % hemolysis, % hematocrit, coagulation time, pH, electrolyte concentrations and degree of blood cell lipid peroxidation by the anti-oxidation mechanism. Skin fibroblast in vitro cell migration result suggests that AgcPCNPs enhanced the degree of cell movement towards the wound area. Data obtained collectively demonstrate that AgcPCNPs can be a better agent in the dermal wound healing with reduced toxicity with the bi-phasic advantage of cPC as a wound healer and Ag nano-metal as an anti-bacterial agent.