Protein-protein interactions in eukaryotic transcription initiation: Structure of the preinitiation complex
Protein-protein interactions in eukaryotic transcription initiation: Structure of the preinitiation complex
复制标题
DOI：
10.1073/pnas.93.3.1119
复制
发表时间：
1996-02-06
影响因子：
11.1
通讯作者：
Ebright, RH
中科院分区：
文献类型：
--
作者：
Tang, H;Sun, XQ;Ebright, RH
已经使用丙氨酸扫描来分析转录前起始复合物中的人TATA元件结合蛋白（TBP）的蛋白质-蛋白质相互作用。结果表明，TBP与RNA聚合酶II和一般的转录因子IIA，IIB和IIF的功能性转录前起始复合物内的相互作用，并定义这些相互作用的TBP的决定因素。结果允许构建一个模型的preinitiation复杂的结构。
have used alanine scanning to analyze protein-protein interactions by human TATA-element binding protein (TBP) within the transcription preinitiation complex. The results indicate that TBP interacts with RNA polymerase II and general transcription factors IIA, IIB, and IIF within the functional transcription preinitiation complex and define the determinants of TBP for each of these interactions. The results permit construction of a model for the structure of the preinitiation complex.