Pheromonal interactions among cordate gametophytes of the lady fern, Athyrium filix-femina
Pheromonal interactions among cordate gametophytes of the lady fern, Athyrium filix-femina
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DOI：
10.1640/0002-8444(2004)094
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发表时间：
2004-01-01
影响因子：
0.8
通讯作者：
Curry, D
中科院分区：
文献类型：
--
作者：
Greer, GK;Curry, D
采用一种通常用于检测水溶性信息素（如花青素）的方法，对雌性蕨类植物a . filix-femina的果核配子体之间的信息素相互作用进行了分析。三周大的果枣配子体从营养琼脂上培养的多孢子培养基转移到含有上一代配子体提取物的琼脂（处理）和新鲜营养琼脂（对照）上。移植3周后，从处理板和对照板上检测50个配子体。测量每个配子体的大小（面积）和形状（圆度），并对精子和颈卵器的数量进行评分。与对照配子体相比，处理后的配子体明显更小，圆形更少，有更少的原卵器，并且具有更多的孢子，这与已知的孢子素对配子体的过渡形态和敏感性的影响是一致的。将6周大的配子体转移到处理板和对照板上重复实验。在第二个实验中，处理配子体的大小（面积）和长度与对照配子体没有显著差异；而处理后的配子体更圆，具有更大的宽度和长度：宽度比，更深的缺口，更少的顶胞体。我们提出了一个模型，其中一种或多种植物化学物质释放的心形配子体增加了顶端分生组织的背斜分裂率。探讨了这些物质可能是参与源配子体中缺口分生组织和心形形态发育的植物激素，也可能与花青素有关。
Pheromonal interactions between cordate gametophytes of the lady fern, A. filix-femina, were assayed using a protocol typically used for detecting water-soluble pheromones such as antheridiogen. Three week-old, cordate gametophytes were transferred from multispore cultures grown on nutrient agar to agar containing extracts from a previous generation of gametophytes (treatment) and to fresh nutrient agar (control). Three weeks after transfer, fifty gametophytes were examined from treatment and control plates. Each gametophyte was measured for size (area) and shape (circularity) and scored for number of antheridia and archegonia. Treatment gamotophytes were significantly smaller, less circular, had fewer archegonia, and possessed antheridia more often than control gametophytes, a pattern consistent with known antheridiogen effects on gametophytes of transitional morphology and sensitivity. The experiment was repeated using gametophytes that were six weeks old at time of transfer to treatment and control plates. Treatment gametophytes in the second experiment did not differ significantly in size (area) or length from control gametophytes; however treatment gametophytes were more circular and possessed greater widths and length : width ratios, deeper notches, and fewer archegonia. We present a model in which one or more phytochemicals released by cordate gametophytes increase rates of anticlinal division in the apical meristem. The possibilities that the substances involved are phytohormones involved in the development of a notch meristem and cordate morphology in the source gametophyte, and that antheridiogen may be involved, are explored.