Main flavonoids in the root of Scutellaria baicalensis cultivated in Europe and their comparative antiradical properties
Main flavonoids in the root of Scutellaria baicalensis cultivated in Europe and their comparative antiradical properties
复制标题
DOI：
10.1002/ptr.1216
复制
发表时间：
2003-06-01
影响因子：
7.2
通讯作者：
Táborská, E
中科院分区：
文献类型：
--
作者：
Bochoráková, H;Paulová, H;Táborská, E
采用流动相乙腈梯度反相高效液相色谱法测定了中欧栽培黄芩根中主要黄酮类化合物的含量。黄芩根的主要成分为黄芩苷（占干根质量的8.12%）和枸杞苷（占干根质量的2.52%）。黄酮类化合物的含量与天然栽培植物的含量相当。研究了5种主要黄酮类化合物对DPPH自由基生成系统的清除能力，但由于溶解度有限，仅研究了2种黄酮类化合物对羟基自由基的清除能力。总提取物也在两种实验安排下进行了测试。在DPPH实验中，只有黄芩苷和黄芩苷表现出明显的清除作用，而黄芩苷和枸杞苷葡糖苷存在时，紫外光解H2O2产生的OH自由基的产生明显减少。通过与总提取物的比较，认为黄芩苷对DPPH的清除作用主要来源于黄芩苷。另一方面，黄芩苷、枸杞苷、葡萄糖醛酸盐和其他类黄酮可能参与清除OH自由基。版权所有：John Wiley Sons， Ltd。
The content of the main flavonoids in the root of Scutellaria baicalensis Georgi cultivated in Central Europe was evaluated using the new simple RP-HPLC method with gradient of acetonitrile in mobile phase. The main components of the roots were baicalin (8.12% of dry root mass) and wogonin glucuronide (2.52%). The content of flavonoids was comparable with the content in plants cultivated in natural localities. Five main flavonoids were evaluated for their scavenging ability with DPPH radical-generating system and due to limited solubility only two flavonoids were investigated for their ability to scavenge hydroxyl radical by the aromatic hydroxylation method. The total extract was also tested in both the experimental arrangements. In experiments with DPPH, only baicalin and baicalein displayed a significant scavenging effect, while the production of OH radicals generated by UV photolysis of H2O2 was considerable decreased in the presence of baicalin and wogonin glucuronide. After comparison with results obtained for the total extract, it was concluded, that the scavenging activity of the extract against DPPH is mainly derived from baicalin. On the other hand, baicalin, wogonin glucuronide and probably other flavonoids participate in scavenging OH radical. Copyright (C) 2003 John Wiley Sons, Ltd.