Characterization of Tpn1 family in the Japanese morning glory:: En/Spm-related transposable elements capturing host genes
Characterization of Tpn1 family in the Japanese morning glory:: En/Spm-related transposable elements capturing host genes
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DOI：
10.1093/pcp/pch109
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发表时间：
2004-07-01
影响因子：
4.9
通讯作者：
Nitasaka, E
中科院分区：
文献类型：
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作者：
Kawasaki, S;Nitasaka, E
由于日本牵牛花（iomoea nil）中的转座因子的插入，一些突变表型在生理上和生发上都是不稳定的。最近已经分离出几个引起可变表型的转座因子。所有这些元件都表现出En/Spm（增强子/抑制子-突变子）或CACTA家族的特征。它们携带共同的28bp末端反向重复和亚末端重复区域，被称为Tpn1家族。所有这些元素都被认为是非自治的，并被未识别的自治元素所动员。利用亚末端区对应的探针，我们分离了多个基因组Tpn克隆，根据其限制性内切图谱将120个Tpn克隆划分为28个类型。据估计，Tpn1家族的拷贝数在每个单倍体基因组500到1000个拷贝之间。然后，我们确定了每种Tpn类型的28个代表性克隆的完整序列。大多数Tpn元件的内部序列与植物基因高度相似，表明Tpn1家族在进化过程中捕获了宿主基因序列。对Tpn104与一个同源宿主基因InAP2B的详细分析证实了这一假设。
Some mutant phenotypes are known to be unstable somatically and germinally due to the insertion of transposable elements in the Japanese morning glory (Ipomoea nil). Several transposable elements that cause mutable phenotypes have recently been isolated. All of these elements show characteristic features of the En/Spm (Enhancer/Suppressor-mutator) or CACTA family. They carry common 28 bp terminal inverted repeats and subterminal repetitive regions and are known as the Tpn1 family. All of these elements are thought to be non-autonomous and mobilized by unidentified autonomous element(s). Using a probe corresponding to the subterminal region, we isolated many genomic Tpn clones, 120 of which were classified into 28 types based on their restriction maps. The copy number of the Tpn1 family was estimated to be between 500 and 1,000 copies per haploid genome. We then determined the complete sequences of 28 representative clones from each Tpn type. Most Tpn elements showed a high degree of similarity to plant genes in their internal sequences, suggesting that the Tpn1 family captured host gene sequences during the process of evolution. Detailed analyses of Tpn104 in comparison with an orthologous host gene InAP2B confirmed this assumption.