Refilling of ocular lens capsule with copolymeric hydrogel containing reversible disulfide
Refilling of ocular lens capsule with copolymeric hydrogel containing reversible disulfide
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DOI：
10.1021/bm049574c
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发表时间：
2005-01-01
影响因子：
6.2
通讯作者：
Ravi, N
中科院分区：
文献类型：
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作者：
Aliyar, HA;Hamilton, PD;Ravi, N
聚合物溶液可以使用微创技术填充任何潜在的不规则腔，因此在眼科学中具有潜在的用途。我们在乙醇水溶液中通过自由基聚合制备了含有二硫键的丙烯酰胺水凝胶。使用二硫苏糖醇将水凝胶液化以产生含有侧链硫醇（-SH）基团的水溶性丙烯酰胺共聚物。通过GPC测得共聚物的重均分子量为1.43 × 10（5）至9.22 × 10（5）道尔顿。Ellman的分析和拉曼光谱证实了-SH的存在。将这些纯化的含硫醇共聚物的水溶液用3，3 ′-二硫代二丙酸或空气氧化以改革水凝胶。根据浓度和硫醇含量，改性水凝胶的模量范围为0.27至1.1 kPa。快速囊内凝胶化在透镜囊袋内产生光学透明的凝胶。这项技术现在使我们能够验证方法，以确定生物力学的透镜和它的作用，在调节。
Polymer solutions can fill any potential irregular cavity using minimally invasive techniques and thus have potential uses in ophthalmology. We prepared acrylamide hydrogels containing disulfide bonds by free radical polymerization in aqueous ethanol. The hydrogels were liquefied using dithiothreitol to yield water-soluble acrylamide copolymers containing pendant thiol (-SH) groups. The weight average molecular weights of the copolymers ranged from 1.43 x 10(5) to 9.22 x 10(5) daltons by GPC. Ellman's analysis and Raman spectroscopy confirmed the presence of -SH. The aqueous solutions of these purified thiol-containing copolymers were oxidized with 3,3'-dithiodipropionic acid or air to reform the hydrogels. The moduli of the reformed hydrogels ranged from 0.27 to 1.1 kPa depending on concentration and thiol content. Rapid endocapsular gelation yielded optically clear gel within the lens capsular bag. This technique now enables us to validate methods to determine the biomechanics of the lens and its role in accommodation.