Targeted high-resolution ion mobility separation coupled to ultrahigh-resolution mass spectrometry of endocrine disruptors in complex mixtures.
Targeted high-resolution ion mobility separation coupled to ultrahigh-resolution mass spectrometry of endocrine disruptors in complex mixtures.
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DOI：
10.1021/ac504866v
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发表时间：
2015-04-21
影响因子：
7.4
通讯作者：
Fernandez-Lima F
中科院分区：
文献类型：
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作者：
Benigni P;Thompson CJ;Ridgeway ME;Park MA;Fernandez-Lima F
由于化学成分数量众多且结构多样性（高度异构），从环境基质的复杂混合物中传统分离和检测目标化合物需要使用冗长的预分馏步骤和高分辨率质量分析仪。在目前的工作中，选择累积捕获离子迁移谱 (SA-TIMS) 与傅里叶变换离子回旋共振质谱 (FT-ICR MS) 联用，可在单次分析中从复杂的环境基质中直接分离和表征目标内分泌干扰化合物 (EDC)。特别是，基于高分辨率迁移率 (R ~ 70–120) 和超高分辨率质量测量 (R > 400 000) 对七种常见目标 EDC 及其同工酶（例如双酚 A、(Z)- 和 (E)-己烯雌酚、己烯雌酚、雌酮、α-雌二醇和 17-乙炔雌二醇）的复杂混合物进行了目标鉴定。水溶性有机物（例如 Suwannee 河黄腐酸标准 II）辅以参考标准测量和理论计算（<3% 误差）。
Traditional separation and detection of targeted compounds from complex mixtures from environmental matrices requires the use of lengthy prefractionation steps and high-resolution mass analyzers due to the large number of chemical components and their large structural diversity (highly isomeric). In the present work, selected accumulation trapped ion mobility spectrometry (SA-TIMS) is coupled to Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) for direct separation and characterization of targeted endocrine-disrupting compounds (EDC) from a complex environmental matrix in a single analysis. In particular, targeted identification based on high-resolution mobility (R ~ 70–120) and ultrahigh-resolution mass measurements (R > 400 000) of seven commonly targeted EDC and their isobars (e.g., bisphenol A, (Z)- and (E)-diethylstilbestrol, hexestrol, estrone, α-estradiol, and 17-ethynylestradiol) is shown from a complex mixture of water-soluble organic matter (e.g., Suwannee River Fulvic Acid Standard II) complemented with reference standard measurements and theoretical calculations (<3% error).