18-P014 ChickATLAS: A three-dimensional atlas of gene expression during chick development
18-P014 ChickATLAS: A three-dimensional atlas of gene expression during chick development
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18-P014 ChickATLAS：小鸡发育过程中基因表达的三维图谱
DOI：
10.1016/j.mod.2009.06.781
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发表时间：
2009
影响因子：
2.6
通讯作者：
Wong F
中科院分区：
文献类型：
--
作者：
Wong F
基因表达调控在胚胎发育中具有重要意义。定量分析，如微阵列，提供了大量基因的基因表达水平，但空间分辨率较低。相比之下，原位杂交和免疫组织化学等原位方法提供了高空间分辨率，但量化较差，基因组覆盖率较低。此外，关键的3D数据在平面样本中会丢失。光学投影断层成像(OPT)可以以合理的高分辨率和适度的高通量捕捉整个胚胎的完整3D表达模式。一个包含老鼠(Emage)时空表达模式的大型数据库已经创建，并被证明是一个宝贵的资源。最近，雏鸡已经成为空间和时间控制功能增益和损失方法的重要模型。到目前为止，还没有建立起完善的雏鸡基因表达数据库。因此，本项目的目的是制作一份鸡胚胎的3D解剖图谱和本体论，并建立一个关于鸡发育过程中基因表达模式的数据库。这涉及爱丁堡、都柏林、巴斯和伦敦的团体之间的重大合作。这个数据库将以Emage为基础，并通过同源基因对(http://www.)与小鼠交叉引用电子地图集。Org/Testemage/home。Php)。在整个项目中，数据和框架将被用来识别在重要信号区域共表达的基因组。这些基因的保守性将在小鸡和小鼠身上进行检查。该数据库将向公众开放(http://www.回声地图集。Org/)，并将成为发展界的宝贵资源。
The control of gene expression is important in embryonic development. Quantitative assays, such as microarrays, provide gene expression levels for large numbers of genes, but with low spatial resolution. In contrast, in situ methods, such as in situ hybridisation and immunohistochemistry, provide high spatial resolution, but poorer quantification and low genome coverage. Furthermore, crucial 3D data is lost with planar samples. Optical projection tomography (OPT) can capture the full 3D expression pattern in a whole embryo at a reasonably high resolution and at moderately high throughput. A large database containing spatio-temporal patterns of expression for the mouse (EMAGE) has been created and is proving to be a valuable resource. Recently, the chick has become an important model for spatially and temporally controlled gain-and loss-of-function approaches. To date, a well-established gene expression database for the chick does not exist. Thus, the aim of this project is to produce a 3D anatomical atlas and ontology of the chick embryo with a database of gene expression patterns during chick development. This involves a major collaboration between groups in Edinburgh, Dublin, Bath and London. This database will be based on EMAGE and cross-referenced to the mouse through orthologous gene pairs (http://www. emouseatlas. org/testemage/home. php). Throughout this project, the data and framework will be used to identify groups of genes that are co-expressed in important signalling regions. Conservation of these genes will be examined in the chick and mouse. This database will be made publicly available (http://www. echickatlas. org/) and will be a valuable resource to the developmental community.